用于分化小鼠3T3-L1和SVF来源的前脂肪细胞并分离粗线粒体组分的实验方案。

Protocol for differentiating murine 3T3-L1 and SVF-derived preadipocytes and isolating crude mitochondrial fractions.

作者信息

Wisessaowapak Churaibhon, Lee Jeongmin, Kim Hyeonhui, Son Seunghwan, Feng Xue, Chang Lina, Hoang Annie, Chen Hetty, Bedsted Sarah, Saltiel Alan R

机构信息

Division of Endocrinology and Metabolism, Department of Medicine, University of California, San Diego, San Diego, CA, USA.

出版信息

STAR Protoc. 2025 Aug 23;6(3):104045. doi: 10.1016/j.xpro.2025.104045.

DOI:10.1016/j.xpro.2025.104045

PMID:40849911

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12398810/

Abstract

Here, we present a protocol for differentiating 3T3-L1 preadipocytes and stromal vascular fraction (SVF)-derived preadipocytes from mice into mature adipocytes, followed by the isolation of crude mitochondrial fractions. This cost-effective and reproducible protocol is optimized for small-plate formats, compatible with standard reagents, and suitable for metabolic studies such as insulin resistance and mitochondrial function.

摘要

在此，我们展示了一种将小鼠的3T3-L1前脂肪细胞和基质血管成分（SVF）衍生的前脂肪细胞分化为成熟脂肪细胞，随后分离粗线粒体组分的方案。这种经济高效且可重复的方案针对小平板形式进行了优化，与标准试剂兼容，适用于胰岛素抵抗和线粒体功能等代谢研究。