Zhao Di, Shi Yifan, Deng Jiatai, Zhong Bolin, Zeng Yuanyuan, Ouyang Qingyuan, Zhang Haihan, Song Zehe, He Xi
College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China; Hunan Engineering Research Center of Poultry Production Safety, Changsha 410128, China; Yuelushan Laboratory, Changsha 410128, China.
College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China; Hunan Engineering Research Center of Poultry Production Safety, Changsha 410128, China; Yuelushan Laboratory, Changsha 410128, China.
Poult Sci. 2025 Aug 14;104(11):105695. doi: 10.1016/j.psj.2025.105695.
While comprehensive transcriptomic characterization of muscle development during the hatching stage remains limited, we conducted an integrated analysis of coding and non-coding RNA profiles in the pectoral muscles of Arbor Acres (AA) broilers and TaoYuan (TY) chickens at embryonic days 17 (E17), 19 (E19), and 21 (E21). Our findings revealed notable phenotypic differences: AA broilers exhibited greater embryo weight and larger muscle fiber cross-sectional areas compared to TY chickens. Across three developmental stages, we identified 4,577 differentially expressed genes (DEGs), 143 differentially expressed microRNAs (DEMs), 90 differentially expressed circRNAs (DECs), and 3,159 differentially expressed lncRNAs (DELs). By integrating weighted gene co-expression network analysis (WGCNA) analysis with differential expression profiling, we prioritized five coding genes (FOSL2, PDE4B, TRIB1, THBS1, and FBXO32) as key regulators of muscle development. Pathway enrichment analysis further revealed significant activation of the glycolysis/gluconeogenesis pathway in TY chickens, likely supporting the energy demands of shell pipping. To elucidate transcriptional regulatory mechanisms, we constructed competing endogenous RNA (ceRNA) networks based on miRanda predictions. This analysis identified four microRNAs (gga-miR-206, gga-miR-383-3p, gga-miR-449a, and gga-miR-449c-5p) that may modulate developmental genes through lncRNA and circRNA mediated sponge interactions. These ceRNA networks provide novel insights and a valuable framework for investigating the molecular regulation of embryonic muscle development in AA broilers and TY chickens.
虽然孵化阶段肌肉发育的全面转录组特征分析仍然有限，但我们对爱拔益加（AA）肉鸡和桃源（TY）鸡胚胎期第17天（E17）、19天（E19）和21天（E21）胸肌中的编码和非编码RNA谱进行了综合分析。我们的研究结果揭示了显著的表型差异：与TY鸡相比，AA肉鸡的胚胎体重更大，肌纤维横截面积更大。在三个发育阶段，我们鉴定出4577个差异表达基因（DEG）、143个差异表达 microRNA（DEM）、90个差异表达环状RNA（DEC）和3159个差异表达长链非编码RNA（DEL）。通过将加权基因共表达网络分析（WGCNA）与差异表达谱分析相结合，我们将五个编码基因（FOSL2、PDE4B、TRIB1、THBS1和FBXO32）确定为肌肉发育的关键调节因子。通路富集分析进一步揭示了TY鸡中糖酵解/糖异生途径的显著激活，这可能支持破壳时的能量需求。为了阐明转录调控机制，我们基于miRanda预测构建了竞争性内源RNA（ceRNA）网络。该分析确定了四个 microRNA（gga-miR-206、gga-miR-383-3p、gga-miR-449a和gga-miR-449c-5p），它们可能通过lncRNA和circRNA介导的海绵相互作用调节发育基因。这些ceRNA网络为研究AA肉鸡和TY鸡胚胎肌肉发育的分子调控提供了新的见解和有价值的框架。
