Hunter Wayne B, Qureshi Jawwad A, Cano Liliana M
Oak Ridge Institute for Science and Education ORISE, Department of Energy and the United States Department of Agriculture DOE/USDA, Agricultural Research Station ARS, Fort Pierce, FL 34945, USA.
United States Department of Agriculture USDA, Agricultural Research Station ARS, U.S. Horticultural Research Laboratory, Fort Pierce, FL 34945, USA.
Microbiome Res Rep. 2025 Apr 3;4(2):19. doi: 10.20517/mrr.2024.84. eCollection 2025.
species are among the most abundant intracellular endosymbionts of insects worldwide. The extensive distribution of Gram-negative among insects highlights their evolutionary success and close relationship with many insect host species. This study aimed to characterize a novel strain from the Wild Lime Psyllid, (), to understand its evolutionary relationship with from psyllid pests like , the vector of Huanglongbing (HLB). Wild-caught colonies from Florida, USA, were maintained on Zanthoxylum fagara. RNA was extracted from the salivary glands, heads, and whole bodies of male and female adult . Four cDNA libraries were sequenced using short read technology and de novo transcriptome assembly was performed. Multilocus sequence typing (MLST) of nine conserved loci and gene analysis classified the strain's phylogeny, while sequence mapping and functional annotation provided insight into host-microbe interactions. The new strain, designated endosymbiont of (wLfag-FL), was assigned to supergroup B, showing relation to strains of other related psyllids. Transcriptome analysis identified 1,359 transcripts with 465 assigned functions encompassing metabolic and secretion system pathways. Ankyrin domain proteins and a partial bacterioferritin sequence were detected, suggesting nutritional provisioning roles. The characterization of wLfag-FL expands the known host range and informs HLB-related pest biology. Its phylogenetic placement and transcript annotations offer insights into symbiotic interactions, potentially guiding environmentally safe pest control strategies targeting psyllid fitness and pathogen transmission.
该菌属是全球昆虫中最丰富的细胞内共生菌之一。革兰氏阴性菌在昆虫中的广泛分布凸显了它们在进化上的成功以及与许多昆虫宿主物种的密切关系。本研究旨在鉴定一种来自野生酸橙木虱（Trioza erytreae）的新型该菌属菌株，以了解其与黄龙病（HLB）传播媒介柑橘木虱（Diaphorina citri）等木虱害虫中该菌属的进化关系。从美国佛罗里达州采集的野生酸橙木虱群体饲养在食茱萸（Zanthoxylum fagara）上。从成年雌雄木虱的唾液腺、头部和全身提取RNA。使用短读长技术对四个cDNA文库进行测序，并进行从头转录组组装。对九个保守位点进行多位点序列分型（MLST）和该菌属基因分析以确定菌株的系统发育，同时序列映射和功能注释有助于了解宿主 - 微生物相互作用。新的该菌属菌株，命名为野生酸橙木虱内共生菌（wLfag - FL），被归入B超群，显示出与其他相关木虱的该菌属菌株有亲缘关系。转录组分析鉴定出1359个该菌属转录本，其中465个具有包括代谢和分泌系统途径在内的指定功能。检测到锚蛋白结构域蛋白和部分细菌铁蛋白序列，表明其具有营养供应作用。wLfag - FL的鉴定扩展了已知的该菌属宿主范围，并为与HLB相关的害虫生物学提供了信息。其系统发育定位和转录注释为共生相互作用提供了见解，可能指导针对木虱适应性和病原体传播的环境安全害虫控制策略。
