species are among the most abundant intracellular endosymbionts of insects worldwide. The extensive distribution of Gram-negative among insects highlights their evolutionary success and close relationship with many insect host species. This study aimed to characterize a novel strain from the Wild Lime Psyllid, (), to understand its evolutionary relationship with from psyllid pests like , the vector of Huanglongbing (HLB). Wild-caught colonies from Florida, USA, were maintained on Zanthoxylum fagara. RNA was extracted from the salivary glands, heads, and whole bodies of male and female adult . Four cDNA libraries were sequenced using short read technology and de novo transcriptome assembly was performed. Multilocus sequence typing (MLST) of nine conserved loci and gene analysis classified the strain's phylogeny, while sequence mapping and functional annotation provided insight into host-microbe interactions. The new strain, designated endosymbiont of (wLfag-FL), was assigned to supergroup B, showing relation to strains of other related psyllids. Transcriptome analysis identified 1,359 transcripts with 465 assigned functions encompassing metabolic and secretion system pathways. Ankyrin domain proteins and a partial bacterioferritin sequence were detected, suggesting nutritional provisioning roles. The characterization of wLfag-FL expands the known host range and informs HLB-related pest biology. Its phylogenetic placement and transcript annotations offer insights into symbiotic interactions, potentially guiding environmentally safe pest control strategies targeting psyllid fitness and pathogen transmission.