低剂量电离辐射与四氯化碳对小鼠肝脏超氧化物歧化酶和谷胱甘肽过氧化物酶的相互作用

Interaction of low doses of ionizing radiation and carbon tetrachloride on liver superoxide dismutase and glutathione peroxidase in mice.

作者信息

Manno M, Bertazzon A, Burlina A, Galzigna L

出版信息

Enzyme. 1985;34(2):107-12. doi: 10.1159/000469370.

Abstract

Male BALB/c mice were treated with intraperitoneal injections of carbon tetrachloride (CCl4) (0.2 g/kg body weight) and/or 50 R of whole-body gamma irradiation, three times per week, for 4 weeks. The effects of the treatments on superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) in liver extracts and homogenates, and on alkaline phosphatase (ALP) in serum were investigated. A significant decrease in the SOD and GSH-Px activities in liver extracts and an increase of serum ALP of hepatic origin were found in CCl4-treated animals. In contrast, only an increase in SOD activity was observed in liver homogenates after the combined treatment.

摘要

雄性BALB/c小鼠每周接受3次腹腔注射四氯化碳（CCl4）（0.2 g/kg体重）和/或50拉德的全身γ射线照射，持续4周。研究了这些处理对肝脏提取物和匀浆中超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GSH-Px）以及血清中碱性磷酸酶（ALP）的影响。在CCl4处理的动物中，发现肝脏提取物中的SOD和GSH-Px活性显著降低，且源自肝脏的血清ALP升高。相比之下，联合处理后肝脏匀浆中仅观察到SOD活性增加。