Dulawa J, Rambausek M, Jann K, Notohamiprodjo M, Ritz E
Diabetologia. 1985 Nov;28(11):827-30. doi: 10.1007/BF00291072.
In the present study, 8 Type 1 diabetic patients with normal creatinine clearance and 8 matched controls were examined. Tamm Horsfall glycoprotein was isolated with the NaCl precipitation procedure. Its purity was checked by gel-electrophoresis, immunodiffusion and isoelectric focussing. Tamm Horsfall glycoprotein of diabetic patients had higher glucose (p less than 0.05) and lower N-acetylneuraminic acid content (p less than 0.01) than controls. 14C-furosemide binding by Tamm Horsfall glycoprotein was examined using an Amicon ultrafiltration system at 0 degree C. In nominally sodium-free medium, furosemide binding by Tamm Horsfall glycoprotein was significantly (p less than 0.01) higher in diabetic patients than in matched controls. The increment of binding capacity with sodium was similar in controls and diabetic patients so that maximal binding capacity in a NaCl system was 1.7 +/- 0.3 in controls and 3.64 +/- 0.5 in diabetic patients (p less than 0.025). Half maximal furosemide binding by Tamm Horsfall glycoprotein occurred at 1.4 +/- 0.2 mmol Na/l in controls and 0.52 +/- 0.12 in diabetic patients (p less than 0.01). Abnormal radiofurosemide binding of Tamm Horsfall glycoprotein of diabetic patients may be the consequence of abnormal postribosomal modification of the glycoprotein which is synthesized in an insulin- and glucose-sensitive nephron segment.
在本研究中，对8名肌酐清除率正常的1型糖尿病患者和8名匹配的对照者进行了检查。采用氯化钠沉淀法分离Tamm-Horsfall糖蛋白。通过凝胶电泳、免疫扩散和等电聚焦检查其纯度。糖尿病患者的Tamm-Horsfall糖蛋白葡萄糖含量较高（p<0.05），N-乙酰神经氨酸含量较低（p<0.01）。在0℃下使用Amicon超滤系统检测Tamm-Horsfall糖蛋白与14C-速尿的结合。在名义上无钠的培养基中，糖尿病患者Tamm-Horsfall糖蛋白与速尿的结合显著高于匹配的对照者（p<0.01）。对照者和糖尿病患者中结合能力随钠的增加相似，因此在氯化钠系统中，对照者的最大结合能力为1.7±0.3，糖尿病患者为3.64±0.5（p<0.025）。Tamm-Horsfall糖蛋白与速尿结合达到最大值一半时，对照者的钠浓度为1.4±0.2mmol/L，糖尿病患者为0.52±0.12（p<0.01）。糖尿病患者Tamm-Horsfall糖蛋白异常的放射性速尿结合可能是在胰岛素和葡萄糖敏感的肾单位段合成的糖蛋白核糖体后修饰异常的结果。
