Akimenko N, Cheltsov P, Balcarová Z, Kleinwächter V, Yevdokimov Y u
Gen Physiol Biophys. 1985 Dec;4(6):597-608.
The optical properties of the DNA complexes with divalent platinum compounds of the cis-diamine type differing both in the nature of anionic and neutral ligands and in the spatial arrangement about the platinum atom were studied. The platinum compounds cis-[Pt(NH3)2Cl2], [Pt(en)Cl2], [Pt(tetrameen)Cl2], cis-[Pt(NH3)2NO2Cl], and cis-[PtNH3(Bz)Cl2] at small values of r (r is the molar ratio of a platinum compound to DNA nucleotides in the reaction mixture) were found to induce an increase in the amplitude of the positive band in the circular dichroic (CD) spectrum of linear DNA. All the compounds listed except cis-[Pt(NH3)2NO2Cl] caused a sharp decrease of the amplitude of the negative band in the CD spectrum of a liquid crystalline microphase of DNA formed in solution in the presence of poly(ethylene glycol). All these platinum compounds (except [Pt(tetrameen)Cl2]) exhibit biological (antimitotic, antitumour, etc.) activity. The platinum compounds trans-[Pt(NH3)Cl2], trans-[Pt(NH3)2NO2Cl], cis-[PtNH3PyCl2], cis-[Pt(NH3)2(NO2)2], and [Pt(NH3)3Cl]Cl exhibiting a low (if any) biological activity, either induced a decrease of the amplitude of the positive band in the CD spectrum of linear DNA, or did not affect the CD spectrum at all. The effect of these platinum compounds on the CD spectrum of the liquid crystalline microphase of DNA was either weak or absent. It is assumed that the specific biological action of platinum compounds of the cis-diamine type is determined by the polydentate binding to DNA: in addition to the cis-bidentate covalent binding of platinum to DNA nitrogen bases, a hydrogen bond formation between the DNA and cis-amino ligands occurs by means of protons at nitrogen atoms.
研究了与顺二胺型二价铂化合物形成的DNA复合物的光学性质，这些化合物在阴离子和中性配体的性质以及铂原子周围的空间排列上均有所不同。发现铂化合物顺式-[Pt(NH₃)₂Cl₂]、[Pt(en)Cl₂]、[Pt(tetrameen)Cl₂]、顺式-[Pt(NH₃)₂NO₂Cl]和顺式-[PtNH₃(Bz)Cl₂]在r值较小时（r是反应混合物中铂化合物与DNA核苷酸的摩尔比）会导致线性DNA圆二色性（CD）光谱中正带振幅增加。除顺式-[Pt(NH₃)₂NO₂Cl]外，列出的所有化合物都会使在聚乙二醇存在下溶液中形成的DNA液晶微相的CD光谱中负带的振幅急剧下降。所有这些铂化合物（除[Pt(tetrameen)Cl₂]外）均表现出生物学（抗有丝分裂、抗肿瘤等）活性。铂化合物反式-[Pt(NH₃)Cl₂]、反式-[Pt(NH₃)₂NO₂Cl]、顺式-[PtNH₃PyCl₂]、顺式-[Pt(NH₃)₂(NO₂)₂]和[Pt(NH₃)₃Cl]Cl表现出低（若有的话）生物学活性，它们要么导致线性DNA的CD光谱中正带振幅下降，要么对CD光谱完全没有影响。这些铂化合物对DNA液晶微相CD光谱的影响要么微弱，要么不存在。据推测，顺二胺型铂化合物的特定生物学作用是由与DNA的多齿结合决定的：除了铂与DNA氮碱基的顺式双齿共价结合外，DNA与顺式氨基配体之间还通过氮原子上的质子形成氢键。
