Byron Michael J, Parker Alison L, Parry Stephen, Merbl Yael
College of Veterinary Medicine, Cornell University Hospital for Animals, Ithaca, New York, USA.
Cornell University Statistical Consulting Unit, Ithaca, New York, USA.
J Vet Intern Med. 2025 Sep-Oct;39(5):e70229. doi: 10.1111/jvim.70229.
Current diagnosis of brain disease in dogs is dependent on imaging and cerebrospinal fluid (CSF) analysis, including total nucleated cell counts and albumin concentrations.
HYPOTHESIS/OBJECTIVES: To determine whether multiplex cytokine/chemokine (Ct/Cm) analysis can differentiate among dogs with meningoencephalitis of unknown origin (MUE), idiopathic epilepsy (IE), and brain neoplasia.
Client owned dogs diagnosed with brain disease with MRI and CSF diagnostics. Groups included 18 dogs with a diagnosis of MUE, 21 dogs with IE, and 7 dogs with brain tumors.
A retrospective observational study; a multiplex immunoassay was utilized to measure CSF concentrations for the following: Interleukin (IL)-2, IL-6, IL-7, IL-8, IL-10, IL-15, and IL-18, granulocyte macrophage colony-stimulating factor (GM-CSF), interferon gamma (IFN-γ), keratinocyte chemoattractant (KC)-like protein, IFN-γ-inducible protein-10 (IP-10), monocyte chemotactic protein 1 (MCP-1), and tumor necrosis factor alpha (TNF-α).
Several Ct/Cm were detected only in MUE cases: GM-CSF (9/18), IFN-γ (13/18), IL-2 (8/18), IL-15 (4/18), and TNF-α (11/18). Other Ct/Cm concentrations were significantly higher in MUE cases (IL-8: median 101 pg/mL, range 144, p = 0.019; IL-18: median 3 pg/mL, range 0.52, p < 0.001; MCP-1: median 814 pg/mL, range 1319, p = 0.004; and IL-6: median 5 pg/mL, range 16, p < 0.001) compared to epilepsy and neoplasia.
IFN-γ, TNF-α, GM-CSF, IL-2, and IL-15 might be specific markers of MUE in canine CSF and could be potentially useful biomarkers in the diagnosis of MUE.
目前犬类脑部疾病的诊断依赖于影像学检查和脑脊液（CSF）分析，包括全有核细胞计数和白蛋白浓度。
假设/目的：确定多重细胞因子/趋化因子（Ct/Cm）分析能否区分不明原因脑膜脑炎（MUE）、特发性癫痫（IE）和脑肿瘤的犬只。
经MRI和CSF诊断确诊患有脑部疾病的客户拥有的犬只。分组包括18只诊断为MUE的犬、21只患有IE的犬和7只患有脑肿瘤的犬。
一项回顾性观察研究；采用多重免疫测定法测量脑脊液中以下物质的浓度：白细胞介素（IL）-2、IL-6、IL-7、IL-8、IL-10、IL-15和IL-18、粒细胞巨噬细胞集落刺激因子（GM-CSF）、干扰素γ（IFN-γ）、角质形成细胞趋化因子（KC）样蛋白、IFN-γ诱导蛋白10（IP-10）、单核细胞趋化蛋白1（MCP-1）和肿瘤坏死因子α（TNF-α）。
仅在MUE病例中检测到几种Ct/Cm：GM-CSF（9/18）、IFN-γ（13/18）、IL-2（8/18）、IL-15（4/18）和TNF-α（11/18）。与癫痫和肿瘤病例相比，其他Ct/Cm浓度在MUE病例中显著更高（IL-8：中位数101 pg/mL，范围144，p = 0.019；IL-18：中位数3 pg/mL，范围0.52，p < 0.001；MCP-1：中位数814 pg/mL，范围1319，p = 0.004；IL-6：中位数5 pg/mL，范围16，p < 0.001）。
IFN-γ、TNF-α、GM-CSF、IL-2和IL-15可能是犬脑脊液中MUE的特异性标志物，可能是诊断MUE的潜在有用生物标志物。
