Munro A D, Pitcher T J
Horm Behav. 1985 Dec;19(4):353-71. doi: 10.1016/0018-506x(85)90034-0.
The effects of three steroid hormones on the agonistic behavior of female Aequidens pulcher have been evaluated. Testosterone, estradiol, and cortisol were tested using an immersion technique to minimize trauma, and we also examined metyrapone, a blocker of cortisol biosynthesis. Two different experimental protocols were employed, the first investigating agonistic interactions within groups of fish, and the second examining the responses of isolated fish to models and mirrors. Differences between replicates were small, and both protocols supported similar conclusions. Each of the three hormones produced a characteristically different spectrum of behaviors when compared to the controls. Testosterone increased agonistic behavior in all experimental situations, while estradiol had a generally opposite effect; this may reflect the natural modulation of behavior by hormones during the reproductive cycle of A. pulcher. Cortisol also had distinct behavioral effects; available evidence suggests that this steroid increases submissive components of agonistic behavior, and that observed increases in some aggressive components are an indirect consequence, dependent upon the feedback of social information received by each fish. Metyrapone treatment greatly reduced all agonistic behaviors, groups of fish forming shoals typical of juveniles. This was not reversed by replacement therapy with cortisol, which suggests that metyrapone affects behavior by an alternative, possibly toxic, mechanism.
已评估了三种类固醇激素对美丽栉丽鱼雌鱼攻击行为的影响。使用浸泡技术来尽量减少创伤，对睾酮、雌二醇和皮质醇进行了测试，并且我们还研究了美替拉酮，一种皮质醇生物合成的阻滞剂。采用了两种不同的实验方案，第一种方案研究鱼群内的攻击相互作用，第二种方案研究隔离的鱼对模型和镜子的反应。重复实验之间的差异很小，两种方案都支持相似的结论。与对照组相比，这三种激素中的每一种都产生了特征性不同的行为谱。睾酮在所有实验情况下都会增加攻击行为，而雌二醇通常具有相反的作用；这可能反映了美丽栉丽鱼繁殖周期中激素对行为的自然调节。皮质醇也有明显的行为效应；现有证据表明，这种类固醇会增加攻击行为中的顺从成分，并且观察到的某些攻击成分的增加是一种间接结果，取决于每条鱼接收到的社会信息的反馈。美替拉酮处理大大减少了所有攻击行为，鱼群形成了典型的幼鱼群。用皮质醇替代疗法并未逆转这种情况，这表明美替拉酮通过一种替代的、可能有毒的机制影响行为。
