INTRODUCTION: Microbial resistance is a growing global concern, necessitating the development of novel drug delivery system to combat the resistant bacterial strains. We aimed to formulate Eudragit based cinnamon essential oil loaded nanoplatform against resistant microbial strain. METHODS: Nanoparticles were characterized for zeta potential, PDI, particle size, SEM, FTIR, entrapment efficiency and drug release kinetic. Box Behnken design with the quadratic model was used to check the effect of independent factors and dependent factors. RESULTS AND DISCUSSION: The and aureus have shown same MIC value of 1.25 μL/ml while and shown 0.078 and 0.625 μL/ml respectively. Quadratic polynomial equation depicted that stirring speed exhibited negative effect on the PDI, particle size and encapsulation efficiency. The polymer concentration produced positive effect on the particle size, PDI and encapsulation efficiency of the nanoparticles. The predicted response values were as particle size (Y1) 228.9 nm, PDI (Y2) 0.3 and %EE (Y3) 72.75% which were very close to the actual values of response as particle size (Y1) was 230.4 ± 3.46 nm, PDI (Y2) was 0.293 ± 0.022, and %EE (Y3) was 74.9 ± 2.32%. It was concluded that our prepared formulation can be effectively used treat resistant bacterial infections.