Sun Yuanfang, Li Shasha, Du Lijing, Ren Xiaopeng, Jin Huizi, Yan Shikai, Xiao Xue
Guangdong Metabolic Diseases Research Center of Integrated Chinese and Western Medicine (Institute of Traditional Chinese Medicine), Guangdong Pharmaceutical University, Guangzhou, China.
School of Pharmacy, Shanghai Jiao Tong University, Shanghai, China.
Front Pharmacol. 2025 Aug 11;16:1647514. doi: 10.3389/fphar.2025.1647514. eCollection 2025.
Microcirculatory dysfunction leads to a number of diseases and worsen prognosis. Blood-activating drugs like capsule may improve circulation, but its pharmacological effects in microcirculatory dysfunction remain unclear.
The purpose of this study is to determine the pharmacological effect of capsules in treating microcirculatory dysfunction.
Adrenaline and icy water stimuli were used to establish a rat model of acute microcirculatory dysfunction. Detecting laser speckle contrast imaging, coagulation function, hemorheology, and the Elisa assay were conducted to investigate the effect of capsule on microcirculation in rats. In addition, the untargeted metabolomics was applied to character the therapeutic effect of systematically from the perspective of endogenous terminal metabolites.
Laser speckle contrast imaging showed that model rats suffered low perfusion in ears, feet and tails, and capsule treatment increased microcirculatory blood flow. capsule diminished the reduction of thrombin time, prothrombin time, activated partial thromboplastin time and the elevated fibrinogen level caused by acute microcirculatory dysfunction. capsule could recover the increased blood viscosity as well as the abnormal vasomotor and microcirculation function in model rats. Furthermore, capsule intervention could alter metabolic state in model rats, which was characterized by the abnormality metabolites mainly in pathway of phospholipids and arachidonic acid metabolism.
The macroscopic image and microscopic indicators elucidated that capsule was highly effective against microcirculatory dysfunction. The present study provided a new perspective on the clinical application of capsule, and contributed to explore novel therapeutic drug against microcirculatory dysfunction.
微循环功能障碍会引发多种疾病并使预后恶化。诸如胶囊之类的活血药物可能会改善血液循环，但其在微循环功能障碍中的药理作用仍不清楚。
本研究旨在确定胶囊在治疗微循环功能障碍中的药理作用。
采用肾上腺素和冰水刺激建立大鼠急性微循环功能障碍模型。通过检测激光散斑对比成像、凝血功能、血液流变学，并进行酶联免疫吸附测定（ELISA）来研究胶囊对大鼠微循环的影响。此外，应用非靶向代谢组学从内源性终末代谢产物的角度系统地表征胶囊的治疗效果。
激光散斑对比成像显示，模型大鼠的耳、足和尾部存在低灌注，而胶囊治疗可增加微循环血流量。胶囊减轻了急性微循环功能障碍所致的凝血酶时间、凝血酶原时间、活化部分凝血活酶时间的缩短以及纤维蛋白原水平的升高。胶囊可恢复模型大鼠升高的血液粘度以及异常的血管舒缩和微循环功能。此外，胶囊干预可改变模型大鼠的代谢状态，其特征为主要在磷脂和花生四烯酸代谢途径中的代谢产物异常。
宏观图像和微观指标表明胶囊对微循环功能障碍具有高效作用。本研究为胶囊的临床应用提供了新的视角，并有助于探索针对微循环功能障碍的新型治疗药物。
