Defilippo Francesco, Gemmellaro M Denise, Grisendi Annalisa, Tranquillo Vito, Lavazza Antonio, Dottori Michele, Moreno Ana
Department of Virology, Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia-Romagna, Brescia, Italy.
Department of Biological Sciences, Kean University, Union, NJ, United States.
Front Insect Sci. 2025 Aug 8;5:1630472. doi: 10.3389/finsc.2025.1630472. eCollection 2025.
The larvae of five dipteran species were reared on artificial diets under controlled laboratory conditions. Usually, these species complete their life cycles in perishable, filthy, unhygienic, and foul-smelling natural diets, which hinder rearing work and affect the laboratory environment. More importantly, these unaltered foods do not allow for true conformity in rearing protocols. The addition of a standard artificial diet to rearing protocols would make it possible to conduct ecological, biological, and forensic investigations with greater accuracy and precision. To address this problem, we formulated a new artificial diet for larvae and tested its performance on five different fly species (, and ). We compared the development of larvae reared on an artificial diet with that of larvae reared on beef liver and pig muscle. The results showed no differences in development time between the two groups. However, our results showed that the artificial diet facilitated the rearing of flies for forensic and medical purposes by standardizing the nutritional value of the diet, improving laboratory conditions, and providing a more hygienic and cost-effective food substrate.
在可控的实验室条件下，用人工饲料饲养了五种双翅目昆虫的幼虫。通常，这些物种在易腐坏、肮脏、不卫生且气味难闻的天然食物中完成其生命周期，这阻碍了饲养工作并影响实验室环境。更重要的是，这些未改变的食物无法使饲养方案真正标准化。在饲养方案中添加标准人工饲料将使生态、生物学和法医调查能够更准确、精确地进行。为解决此问题，我们为幼虫配制了一种新的人工饲料，并在五种不同的蝇类物种（ 、 和 ）上测试了其性能。我们比较了用人工饲料饲养的幼虫与用牛肝和猪肌肉饲养的幼虫的发育情况。结果表明两组之间的发育时间没有差异。然而，我们的结果表明，人工饲料通过使饲料的营养价值标准化、改善实验室条件以及提供更卫生且具成本效益的食物基质，促进了用于法医和医学目的的蝇类饲养。
