Casarella Simona, Ferla Federica, Di Francesco Dalila, Pagani Veronica, Di Varsavia Carolina, Regano Irene, Boccafoschi Francesca
Department of Health Sciences, University of Piemonte Orientale "A. Avogadro", Novara, Italy.
Laboratory for Biomaterials and Bioengineering, Canada Research Chair Tier I for the Innovation in Surgery, Department of Min-Met-Materials Engineering and Regenerative Medicine, CHU de Quebec Research Center, Laval University, Quebec City, QC, Canada.
Front Bioeng Biotechnol. 2025 Aug 8;13:1629412. doi: 10.3389/fbioe.2025.1629412. eCollection 2025.
Laminin represents a major component of the basement membrane in cardiac tissue. Through integrins binding, laminin can sustain cell adhesion, proliferation, differentiation and mechanotransduction. The role of a small bioactive peptide (KKGSYNNIVVHV) derived from laminin in cardiac differentiation and functionality has been demonstrated. Briefly, results showed how the presence of laminin-derived peptide enhanced the differentiation of mesenchymal stem cells into cardiomyocytes-like phenotype, by modulating not only the cytoskeletal apparatus but also enhancing the cardiac and adhesion markers. Moreover, neonatal mouse cardiomyocytes in presence of the peptide showed well organized cytoskeletal rearrangements and regulated contractility. Therefore, in this study the role of laminin-derived peptide in guiding cardiomyogenesis has been characterized, opening innovative approaches in functional cardiac tissue engineering and regenerative medicine.
层粘连蛋白是心脏组织基底膜的主要成分。通过整合素结合，层粘连蛋白可以维持细胞黏附、增殖、分化和机械转导。源自层粘连蛋白的一种小生物活性肽（KKGSYNNIVVHV）在心脏分化和功能方面的作用已得到证实。简而言之，结果表明层粘连蛋白衍生肽的存在通过不仅调节细胞骨架装置而且增强心脏和黏附标记物，促进间充质干细胞向心肌样表型分化。此外，在该肽存在的情况下，新生小鼠心肌细胞表现出良好组织的细胞骨架重排和调节的收缩性。因此，在本研究中，已对层粘连蛋白衍生肽在引导心肌生成中的作用进行了表征，为功能性心脏组织工程和再生医学开辟了创新方法。
