Savu L, Zouaghi H, Nunez E A
Int J Tissue React. 1985;7(6):443-8.
The kinetics of the inflammatory responses of 5 serum parameters involved in specific protein-hormone interactions were studied, namely: transcortin (CBG) binding activities; total corticosterone (B), main ligand of CBG; unbound corticosterone (free B); total progesterone (P4), main competitor of B for binding on CBG; and the free P4. The studies were performed in developing animals, i.e. on rats given a single turpentine injection at 1, 10, 21 or 90 days of post-natal age. The responses showed similar trends at all ages, but age-dependent differences in kinetics and reactivities. Common characteristics were 3-5-fold increases of the hormone parameters as soon as 4-8 h after the injection, and 2-3-fold decreases in CBG activities. A remarkable age-dependent difference was the contrast of early CBG decrease in adults (4 h after turpentine) to the delayed occurrence of this reaction in immatures (8-24 h after turpentine). On the other hand, the increase of free B lasted for 72 h in neonates, but only 40 h in rats treated at 21 or 90 days. Age-dependent characteristics were seen also in reactivities: the peak increases in the hormone levels were highest in the young, while the peak decreases of CBG were most marked in the mature animals. The results suggest that the regulatory mechanisms governing the mutual effects of hormonal activities and CBG synthesis might depend on the stage of development.
研究了参与特定蛋白质-激素相互作用的5种血清参数的炎症反应动力学，具体如下：皮质素转运蛋白（CBG）结合活性；皮质酮总量（B），CBG的主要配体；游离皮质酮（游离B）；孕酮总量（P4），B与CBG结合的主要竞争者；以及游离P4。研究在发育中的动物身上进行，即对出生后1、10、21或90天接受单次松节油注射的大鼠进行研究。所有年龄段的反应都呈现出相似的趋势，但在动力学和反应性方面存在年龄依赖性差异。共同特征是注射后4-8小时内激素参数立即增加3-5倍，CBG活性降低2-3倍。一个显著的年龄依赖性差异是，成年大鼠（松节油注射后4小时）CBG早期下降，而未成熟大鼠（松节油注射后8-24小时）该反应延迟出现。另一方面，游离B的增加在新生儿中持续72小时，但在21天或90天接受治疗的大鼠中仅持续40小时。反应性方面也存在年龄依赖性特征：激素水平的峰值增加在幼龄动物中最高，而CBG的峰值降低在成年动物中最为明显。结果表明，调节激素活性和CBG合成相互作用的调控机制可能取决于发育阶段。
