Wang Yizhen, Zhang Yuyang, Li Yunfei, Cai Fuhong
Key Laboratory of Biomedical Engineering of Hainan Province, School of Biomedical Engineering, Sanya Research Institute, Hainan University, Sanya 572000, China.
College of Electronic and Information Engineering, Hebei University, Baoding 071002, China.
Biosensors (Basel). 2025 Aug 3;15(8):501. doi: 10.3390/bios15080501.
Skin autofluorescence (SAF) detection technology represents a noninvasive, convenient, and cost-effective optical detection approach. It can be employed for the differentiation of various diseases, including metabolic diseases and dermatitis, as well as for monitoring the treatment efficacy. Distinct from diffuse reflection signals, the autofluorescence signals of biological tissues are relatively weak, making them challenging to be captured by photoelectric sensors. Moreover, the absorption and scattering properties of biological tissues lead to a substantial attenuation of the autofluorescence of biological tissues, thereby worsening the signal-to-noise ratio. This has also imposed limitations on the development and application of compact-sized autofluorescence detection systems. In this study, a compact LED light source and a CMOS sensor were utilized as the excitation and detection devices for skin tissue autofluorescence, respectively, to construct a mobile and wireless skin tissue autofluorescence detection system. This system can achieve the detection of skin tissue autofluorescence with a high signal-to-noise ratio under the drive of a simple power supply and a single-chip microcontroller. The detection time is less than 0.1 s. To enhance the stability of the system, a pressure sensor was incorporated. This pressure sensor can monitor the pressure exerted by the skin on the detection system during the testing process, thereby improving the accuracy of the detection signal. The developed system features a compact structure, user-friendliness, and a favorable signal-to-noise ratio of the detection signal, holding significant application potential in future assessments of skin aging and the risk of diabetic complications.
皮肤自体荧光（SAF）检测技术是一种非侵入性、便捷且经济高效的光学检测方法。它可用于多种疾病的鉴别，包括代谢性疾病和皮炎，还可用于监测治疗效果。与漫反射信号不同，生物组织的自体荧光信号相对较弱，这使得光电传感器难以捕捉到它们。此外，生物组织的吸收和散射特性会导致生物组织自体荧光大幅衰减，从而使信噪比恶化。这也给紧凑型自体荧光检测系统的开发和应用带来了限制。在本研究中，分别采用紧凑型发光二极管（LED）光源和互补金属氧化物半导体（CMOS）传感器作为皮肤组织自体荧光的激发和检测装置，构建了一个可移动的无线皮肤组织自体荧光检测系统。该系统在简单电源和单片机的驱动下，能够实现高信噪比的皮肤组织自体荧光检测。检测时间小于0.1秒。为提高系统稳定性，加入了一个压力传感器。该压力传感器可在测试过程中监测皮肤对检测系统施加的压力，从而提高检测信号的准确性。所开发的系统结构紧凑、用户友好，检测信号的信噪比良好，在未来皮肤老化评估和糖尿病并发症风险评估中具有重要的应用潜力。
