Martinez E E, Shimoda W
J Assoc Off Anal Chem. 1985 Nov-Dec;68(6):1149-53.
Monensin sodium does not have an ultraviolet (UV) absorbance above 220 nm, and therefore cannot be detected by liquid chromatography (LC) with a UV detector. A method was developed in which monensin residues are extracted from beef liver tissue, acetylated, partitioned, and reacted with 9-anthryldiazomethane to form a fluorescent derivative for quantitation by LC. The reliable level of sensitivity is 50 ppb, but 15 ppb can be detected. Recoveries ranged between 71 and 96% with an average of 83.5%.
莫能菌素钠在220纳米以上没有紫外吸收，因此不能用带有紫外检测器的液相色谱法进行检测。开发了一种方法，从牛肝组织中提取莫能菌素残留，进行乙酰化、分配，然后与9-蒽重氮甲烷反应形成荧光衍生物，通过液相色谱法定量。可靠的灵敏度水平为50 ppb，但可以检测到15 ppb。回收率在71%至96%之间，平均为83.5%。
