Jinno Y, Matuo S, Nomiyama H, Shimada K, Matsuda I
J Biochem. 1985 Nov;98(5):1395-403. doi: 10.1093/oxfordjournals.jbchem.a135407.
The structure of the 5' end region of the human argininosuccinate synthetase (AS) gene was analyzed in detail. The 5' flanking region up to 350 nucleotides from the putative transcription initiation site is unusually G+C rich (80%). Three repeats of a CCGCCC sequence and one complementary GGGCGG sequence are present in this region. It contains no CCAAT box-like sequence, but does contain one typical TATA box and a 20 bases-long inverted repeat sequence that could form a stem and loop structure just upstream from the TATA box. An octanucleotide sequence, AGAAGTGA, found in the 5' flanking region of the AS gene is also commonly present in those regions of the two other human genes expressed mainly in the liver. These structural features of the AS gene indicate that it shares common structures with two completely different groups of genes, i.e. tissue-specific genes and housekeeping ones. Moreover, two enhancer core-like sequences, alternating purine-pyrimidines tracts and two independent Alu type highly repetitive sequences are apparent in the first intron of the AS gene.
对人精氨琥珀酸合成酶（AS）基因5'端区域的结构进行了详细分析。从假定的转录起始位点起至350个核苷酸的5'侧翼区域富含G+C（80%），情况异常。该区域存在CCGCCC序列的三个重复以及一个互补的GGGCGG序列。它不含类CCAAT盒序列，但含有一个典型的TATA盒和一个20个碱基长的反向重复序列，该序列可在TATA盒上游形成茎环结构。在AS基因5'侧翼区域发现的八核苷酸序列AGAAGTGA，在另外两个主要在肝脏中表达的人类基因的相应区域也普遍存在。AS基因的这些结构特征表明，它与两组完全不同的基因具有共同结构，即组织特异性基因和管家基因。此外，在AS基因的第一个内含子中明显存在两个增强子核心样序列、交替的嘌呤-嘧啶序列以及两个独立的Alu型高度重复序列。
