Cieślik Martyna, Bajrak Olaf, Wójcicki Michał, Orwat Filip, Łodej Norbert, Błażejak Stanisław, Pawlak Edyta, Pawlik Krzysztof J, Górski Andrzej, Jończyk-Matysiak Ewa
Bacteriophage Laboratory, Department of Phage Therapy, Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wrocław, Poland.
Inter-departmental Laboratory of Instrumental Analysis and Preparation, Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wrocław, Poland.
Microbiol Res. 2025 Dec;301:128320. doi: 10.1016/j.micres.2025.128320. Epub 2025 Aug 20.
Due to the rapidly increasing problem of pathogen resistance to drugs, including bacterial resistance to antibiotics, there is a growing need to explore new therapeutic solutions. One of the multidrug-resistant pathogens responsible for many difficult-to-treat infections is Acinetobacter baumannii. The clinical relevance of another species, A. johnsonii, is also increasingly being recognized. As the development pipeline for new antibiotics remains limited, alternative antimicrobial approaches are urgently required. Among these, the therapeutic use of bacteriophages against multidrug-resistant pathogens is currently gaining renewed interest. In this study, we describe the comprehensive characterization of three novel Acinetobacter-specific bacteriophages (Acba_19, Acjo_20 and Acba_21) isolated from sewage samples. The research were conducted using clinical strains obtained from various infection sites of patients in healthcare facilities in Poland. Genomic analysis revealed no markers of temperate phages in any of the isolates, allowing their classification as strictly lytic viruses. In addition to determining basic parameters of phages - such as lytic range, species specificity, virion morphology, adsorption dynamics and infection kinetics - we also conducted stability studies of phage preparations under various conditions. These studies included assessments of pH and temperature stability, evaluation of different cryoprotectants (such as trehalose, glycerol, and pluronic), as well as the effects of storage containers made of glass and plastic materials.
由于包括细菌对抗生素耐药在内的病原体耐药问题迅速增加，探索新的治疗方案的需求日益增长。鲍曼不动杆菌是导致许多难以治疗感染的多重耐药病原体之一。另一种约翰逊不动杆菌的临床相关性也越来越受到认可。由于新抗生素的研发渠道仍然有限，迫切需要替代抗菌方法。其中，噬菌体对多重耐药病原体的治疗应用目前正重新引起人们的兴趣。在本研究中，我们描述了从污水样本中分离出的三种新型不动杆菌特异性噬菌体（Acba_19、Acjo_20和Acba_21）的全面特征。研究使用了从波兰医疗机构患者的各种感染部位获得的临床菌株。基因组分析显示，任何分离株中均未发现温和噬菌体的标记，因此可将它们归类为严格的裂解性病毒。除了确定噬菌体的基本参数，如裂解范围、物种特异性、病毒体形态、吸附动力学和感染动力学外，我们还在各种条件下对噬菌体制剂进行了稳定性研究。这些研究包括对pH值和温度稳定性的评估、对不同冷冻保护剂（如海藻糖、甘油和普朗尼克）的评估，以及玻璃和塑料材料制成的储存容器的影响。
