Ishida J, Kai M, Ohkura Y
J Chromatogr. 1985 Nov 8;344:267-74. doi: 10.1016/s0378-4347(00)82027-7.
A high-performance liquid chromatographic method is described for the fluorimetric determination of p-hydroxybestatin (an active metabolite of bestatin) in human serum. p-Hydroxybestatin is formylated in an alkaline medium in the presence of chloroform, and converted to a fluorescent derivative with 1,2-diamino-4,5-dimethoxybenzene. This derivative is then separated on a reversed-phase column (TSK gel ODS-120T) with isocratic elution. The detection limit of p-hydroxybestatin in serum is 15 ng (46 pmol) per ml serum (115 pg in a 100-microliters injection volume). This method is simple and sensitive enough to determine p-hydroxybestatin in serum (200 microliters) from muscular dystrophic patients and from healthy subjects dosed with bestatin.
本文描述了一种高效液相色谱法，用于荧光测定人血清中的对羟基苯丁抑制素（苯丁抑制素的一种活性代谢物）。对羟基苯丁抑制素在碱性介质中、氯仿存在的条件下被甲酰化，并与1,2 - 二氨基 - 4,5 - 二甲氧基苯转化为荧光衍生物。然后该衍生物在反相柱（TSK凝胶ODS - 120T）上进行等度洗脱分离。血清中对羟基苯丁抑制素的检测限为每毫升血清15 ng（46 pmol）（进样体积100微升时为115 pg）。该方法简便且灵敏，足以测定肌营养不良患者和服用苯丁抑制素的健康受试者血清（200微升）中的对羟基苯丁抑制素。
