紫外线辐射导致大鼠主动脉张力降低及磷酸化酶a生成减少。
Ultraviolet radiation-induced decreases in tension and phosphorylase a formation in rat aorta.
作者信息
Lincoln T M, Laks J, Johnson R M
出版信息
J Cyclic Nucleotide Protein Phosphor Res. 1985;10(6):525-33.
Helical strips of rat aorta contracted with norepinephrine (10 nM) respond to ultraviolet (UV) radiation (340-360 nm) with a transient decrease in tension. The UV radiation-dependent relaxation is completely reversible and endothelial cell-independent. Although decreased tension is associated with a rise in tissue cGMP content, the cAMP level is unchanged after UV radiation. A significant inhibition of phosphorylase a formation which occurs coincidently with the rise in cGMP and decline in tension is observed with UV radiation. The effects of UV radiation on cGMP, phosphorylase a formation and tension were blocked by methylene blue. Relaxation and inhibition of phosphorylase a formation in response to UV radiation were also partially reversed with higher concentrations of norepinephrine (100 nM). Our results suggest that cGMP may mediate UV radiation-dependent reactions by reducing cytoplasmic Ca2+.
用去甲肾上腺素（10 nM）收缩的大鼠主动脉螺旋条对紫外线（UV，340 - 360 nm）产生反应，张力会短暂降低。依赖紫外线辐射的舒张是完全可逆的且不依赖内皮细胞。尽管张力降低与组织中环鸟苷酸（cGMP）含量升高相关，但紫外线辐射后环磷酸腺苷（cAMP）水平未变。紫外线辐射时可观察到磷酸化酶a形成受到显著抑制，这与cGMP升高和张力降低同时发生。紫外线辐射对cGMP、磷酸化酶a形成和张力的影响被亚甲蓝阻断。对紫外线辐射的舒张反应及磷酸化酶a形成的抑制，在较高浓度去甲肾上腺素（100 nM）作用下也会部分逆转。我们的结果表明，cGMP可能通过降低细胞质钙离子（Ca2+）来介导依赖紫外线辐射的反应。
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