Hase H
Nihon Seikeigeka Gakkai Zasshi. 1985 Aug;59(8):785-801.
Ultrastructural studies were carried out to examine the normal structure and postmortem autolytic changes of the cells and matrix of nucleus pulposus using adult monkey. Two kinds of cells were observed in the nucleus pulposus. The one was chondrocyte, which contained normal organelles and that was characterized by large halo. The halo was composed of numerous "crista like structures", that were regarded to form the matrix of nucleus pulposus. The other was notochordal cell, most of which appeared in grouping or separately, and yet had cell activity. In addition, there were the intermediate type of cells between chondrocyte and notochordal cell. The ultrastructural autolytic changes were rarely seen in the cells of 6 hours after death, but the changes in the halo and in the cytoplasm were remarkable after more than 12 hours. The autopsied nucleus pulposus for electron microscopical examination should be used within 6 hours after death in usual room temperature.
采用成年猴子进行超微结构研究，以检查髓核细胞和基质的正常结构以及死后自溶变化。在髓核中观察到两种细胞。一种是软骨细胞，其含有正常细胞器，特征是有大的晕环。该晕环由众多“嵴样结构”组成，这些结构被认为构成了髓核的基质。另一种是脊索细胞，大多数呈成群或单个出现，且具有细胞活性。此外，在软骨细胞和脊索细胞之间还有中间类型的细胞。死后6小时内细胞的超微结构自溶变化很少见，但12小时以上后晕环和细胞质中的变化很明显。在常温下，用于电子显微镜检查的尸检髓核应在死后6小时内使用。
