Hirota Masatsugu, Yamamoto Takatsugu
Department of Education for Dental Medicine, Tsurumi University School of Dental Medicine, 2-1-3, Tsurumi, Tsurumi-ku, Yokohama 230-8501, Japan.
Department of Operative Dentistry, Tsurumi University School of Dental Medicine, 2-1-3, Tsurumi, Tsurumi-ku, Yokohama 230-8501, Japan.
Materials (Basel). 2025 Aug 18;18(16):3856. doi: 10.3390/ma18163856.
Protein adsorption on dental zirconia (ZrO) surfaces plays a crucial role in plaque formation, tissue healing, and bone osseointegration. This study investigated and compared the adsorption behavior of three salivary antimicrobial proteins-peroxidase, lactoferrin, and lysozyme-on a ZrO sensor and an Au sensor using a quartz crystal microbalance (QCM) operating at 27 MHz. Protein adsorption was determined from frequency decreases, and the apparent reaction rate constant () was calculated by fitting frequency-time curves to a kinetic model. The amount of lactoferrin adsorbed on the ZrO sensor was significantly higher than that of peroxidase and lysozyme ( < 0.05). Significantly smaller amounts of peroxidase and lysozyme were adsorbed onto the ZrO sensor than the Au sensor ( < 0.05). The for lysozyme on the Au sensor was significantly higher than those for lactoferrin on sensors and for peroxidase on the Au sensor ( < 0.05). Differences in salivary antimicrobial protein adsorption between Au and ZrO surfaces were influenced, in part, by electrostatic interactions between the proteins and the material surface.
蛋白质在牙科氧化锆（ZrO）表面的吸附在菌斑形成、组织愈合和骨整合中起着至关重要的作用。本研究使用工作频率为27MHz的石英晶体微天平（QCM），研究并比较了三种唾液抗菌蛋白——过氧化物酶、乳铁蛋白和溶菌酶——在ZrO传感器和金传感器上的吸附行为。通过频率下降来确定蛋白质吸附量，并通过将频率-时间曲线拟合到动力学模型来计算表观反应速率常数（）。吸附在ZrO传感器上的乳铁蛋白量显著高于过氧化物酶和溶菌酶（<0.05）。吸附在ZrO传感器上的过氧化物酶和溶菌酶量明显少于金传感器（<0.05）。溶菌酶在金传感器上的明显高于乳铁蛋白在传感器上的以及过氧化物酶在金传感器上的（<0.05）。金和ZrO表面之间唾液抗菌蛋白吸附的差异部分受蛋白质与材料表面之间静电相互作用的影响。
