Department of Dental Engineering, Tsurumi University School of Dental Medicine, Kanagawa, Japan.
Biomed Mater Eng. 2022;33(1):1-11. doi: 10.3233/BME-206013.
The adsorption of salivary pellicle proteins onto the material surface is key for denture plaque formation.
We aimed to investigate the adsorption of bovine serum albumin (BSA) and mucin (MCN) onto denture base metal materials using a 27-MHz quartz crystal microbalance (QCM) method.
A gold (Au), titanium (Ti), and cobalt chromium alloy (Co-Cr) sensors were employed for QCM measurements. Adsorbed amounts of BSA or MCN were calculated by observing the frequency decrease, and the apparent reaction rate, kobs, was obtained by the curve fitting of the frequency shift against the adsorption time.
The adsorbed amounts of BSA on Ti were significantly lower than those on Au and Co-Cr. For MCN adsorption, Au showed significantly greater amounts of adsorption than Co-Cr. The kobs of Ti for BSA adsorption was significantly smaller than for the Co-Cr. The kobs of Ti, and Co-Cr for MCN adsorption were significantly smaller than for the Au. A clear correlation was not determined between adsorbed amounts of BSA or MCN onto each sensor and the surface topography or contact angles.
The difference of denture base metals and the difference of salivary proteins influences the adsorption behavior of salivary proteins.
唾液膜蛋白吸附到材料表面是义齿菌斑形成的关键。
我们旨在使用 27MHz 石英晶体微天平（QCM）方法研究牛血清白蛋白（BSA）和黏蛋白（MCN）在义齿基托金属材料上的吸附。
采用金（Au）、钛（Ti）和钴铬合金（Co-Cr）传感器进行 QCM 测量。通过观察频率降低来计算 BSA 或 MCN 的吸附量，并通过对频率偏移与吸附时间的曲线拟合来获得表观反应速率 kobs。
Ti 上 BSA 的吸附量明显低于 Au 和 Co-Cr。对于 MCN 的吸附，Au 的吸附量明显大于 Co-Cr。Ti 对 BSA 吸附的 kobs 明显小于 Co-Cr。Ti 和 Co-Cr 对 MCN 吸附的 kobs 明显小于 Au。BSA 或 MCN 吸附量与每个传感器的表面形貌或接触角之间未确定明确的相关性。
义齿基托金属的差异和唾液蛋白的差异影响唾液蛋白的吸附行为。
