Yamauchi A, Nakayama A, Kariya K
J Pharmacobiodyn. 1985 Aug;8(8):607-13. doi: 10.1248/bpb1978.8.607.
Kinin level in the rat brain was determined using a highly sensitive radioimmunoassay with a purified tracer. Monoiodinated Tyr8-bradykinin (BK) was separated from the non-iodinated peptide by paper chromatography, using 0.2 M ammonium acetate as the developer. This purification method resulted in a ligand with a highly specific radioactivity (2200 muCi/nmol), which raised the radioimmunoassay sensitivity about 5-fold and enabled to determine the brain kinin level in the rat. The brain extract competed with iodinated antigen for an antibody binding site in the same manner as did authentic BK, indicating the immunological identity of them. Brain kinin level was estimated to be 139 +/- 79 fmol BK eq/g (n = 7) in an adult male rat of the Sprague-Dawley strain.
采用高度灵敏的放射免疫分析法和纯化的示踪剂测定大鼠脑中的激肽水平。使用0.2M醋酸铵作为展开剂，通过纸色谱法将单碘化的Tyr8-缓激肽（BK）与未碘化的肽分离。这种纯化方法得到了一种具有高比放射性（2200μCi/nmol）的配体，这使放射免疫分析的灵敏度提高了约5倍，并能够测定大鼠脑中的激肽水平。脑提取物与碘化抗原竞争抗体结合位点的方式与天然BK相同，表明它们在免疫学上具有同一性。在成年雄性Sprague-Dawley品系大鼠中，脑激肽水平估计为139±79 fmol BK当量/g（n = 7）。
