Alanagreh Lo'ai, Mustafa Minas A, Al-Zghoul Mohammad Borhan, Massadeh Muhannad I, Batiha Osamah, Sughayer Maher, Almashakbeh Rashed Taiseer, Abu Suilike Haya Bader, Tout Faten S, Alzoughool Foad
Department of Medical Laboratory Sciences, Faculty of Applied Medical Sciences, The Hashemite University, Zarqa 13133, Jordan.
Department of Medical Laboratory, Faculty of Allied Medical Sciences, Zarqa University, Zarqa 13110, Jordan.
Microorganisms. 2025 Aug 21;13(8):1963. doi: 10.3390/microorganisms13081963.
The gut microbiota plays a pivotal role in developing colorectal cancer (CRC) through interactions with host immunity, metabolism, and inflammation. However, microbiome-based studies remain scarce in Middle Eastern populations, limiting regional insights into microbial signatures associated with CRC. This study aimed to characterize the gut microbiota profiles of Jordanian CRC patients using 16S rRNA gene sequencing and compare them to those of healthy controls from the GutFeeling KnowledgeBase (GutFeelingKB). Stool samples from 50 CRC patients were analyzed using Illumina iSeq targeting the V3-V4 region. Taxonomic profiling was conducted with a standardized 16S metagenomics pipeline and compared with GutFeelingKB reference data. CRC samples were enriched in , , , , , , , and , while beneficial butyrate-producing genera such as , , , and were significantly depleted. The absence of and -commonly seen in global studies-suggests region-specific microbial patterns. This study is the first metagenomic study profiling CRC-associated microbiota in Jordan. The findings reveal a dysbiotic microbial signature that reflects both global changes associated with CRC and local ecological influences. This research emphasizes the importance of population-specific microbiome studies and highlights the need to include appropriately matched controls in future investigations.
肠道微生物群通过与宿主免疫、代谢和炎症相互作用，在结直肠癌（CRC）的发生发展中起关键作用。然而，基于微生物组的研究在中东人群中仍然很少，限制了对与CRC相关的微生物特征的区域见解。本研究旨在利用16S rRNA基因测序对约旦CRC患者的肠道微生物群谱进行表征，并将其与来自肠道感觉知识库（GutFeelingKB）的健康对照进行比较。使用Illumina iSeq靶向V3-V4区域对50名CRC患者的粪便样本进行分析。采用标准化的16S宏基因组学流程进行分类分析，并与GutFeelingKB参考数据进行比较。CRC样本中 、 、 、 、 、 、 和 富集，而有益的产丁酸盐菌属如 、 、 和 则显著减少。在全球研究中常见的 和 的缺失表明存在区域特异性微生物模式。本研究是约旦第一项对与CRC相关的微生物群进行宏基因组分析的研究。研究结果揭示了一种失调的微生物特征，既反映了与CRC相关的全球变化，也反映了局部生态影响。本研究强调了针对特定人群的微生物组研究的重要性，并突出了在未来研究中纳入适当匹配对照的必要性。
