Liu Zidan, Luo Linyu, Ding Zhiqiang, Long Weihao, Osire Tolbert, Li Qiong, Chen Qianfeng, Long Mengfei
College of Pharmaceutical Sciences, Southwest University, Chongqing 400715, China.
Faculty of Biology, Shenzhen MSU-BIT University, Shenzhen 518172, China.
Molecules. 2025 Aug 19;30(16):3416. doi: 10.3390/molecules30163416.
Stevioside, a natural high-intensity sweetener, is widely employed across the food, pharmaceutical, and daily chemical industries due to its intense sweetness and health benefits. However, traditional extraction and purification processes for steviol glycosides from are plagued by low efficiency, high energy consumption, substantial environmental impact, and inconsistent product quality. This study systematically optimized the extraction, decolorization, decontamination, and desalting processes to overcome these challenges. The extraction method was refined using 20% ethanol as the solvent, an optimal temperature of 50 °C, and a 1:10 material-to-liquid ratio, increasing the steviol glycoside yield from 32.0% to 49.1%. Decolorization employing a combination of resins D940 and T5 achieved decolorization rates of 89-92% with minimized steviol glycoside loss, surpassing the non-selective adsorption limitations of activated carbon. For decontamination, calcium hydroxide (Ca(OH)) outperformed diatomaceous earth, attaining a 98% protein removal rate while maintaining steviol glycoside loss below 20%. The desalting resin LXP-016 demonstrated superior performance at 40 °C, enhancing the ability of ionic impurity removal. These optimizations collectively improve the efficiency, sustainability, and quality of steviol glycoside production, offering a promising framework for industrial-scale applications.
甜菊糖苷是一种天然高强度甜味剂，因其甜度高且有益健康，在食品、制药和日用化工行业广泛应用。然而，从甜叶菊中提取和纯化甜菊糖苷的传统工艺存在效率低、能耗高、对环境影响大以及产品质量不稳定等问题。本研究系统地优化了提取、脱色、除杂和脱盐工艺，以克服这些挑战。提取方法优化为使用20%乙醇作为溶剂，最佳温度为50℃，料液比为1:10，甜菊糖苷得率从32.0%提高到49.1%。采用D940和T5树脂组合进行脱色，脱色率达到89 - 92%，甜菊糖苷损失最小，克服了活性炭非选择性吸附的局限性。除杂方面，氢氧化钙（Ca(OH)）优于硅藻土，蛋白质去除率达到98%，同时甜菊糖苷损失保持在20%以下。脱盐树脂LXP - 016在40℃时表现出优异性能，提高了去除离子杂质的能力。这些优化共同提高了甜菊糖苷生产的效率、可持续性和质量，为工业规模应用提供了一个有前景的框架。
