Moisi Răzvan, Comănescu Mircea-Alexandru, Medvedovici Andrei-Valentin
Department of Analytical & Physical Chemistry, Faculty of Chemistry, University of Bucharest, Panduri Ave. #90-92, 050663 Bucharest, Romania.
Molecules. 2025 Aug 21;30(16):3443. doi: 10.3390/molecules30163443.
Nicotine is a highly used addictive substance that has recently also become available through electronic cigarettes. Here we present a study of nicotine from e-cigarette liquids through reversed-phase (RP) and hydrophilic interaction (HILIC) liquid chromatography. Multiple aqueous mobile-phase additives are considered for the RP mechanism, focusing on chaotropic agents, mobile-phase concentrations and mixing ratios, and column temperature. Sample preparation was conducted by toluene liquid-liquid extraction of e-cigarette liquids diluted with aqueous 25 mM NaHCO/NaCO. Optimal RP results for retention and peak symmetry were obtained using aqueous 0.1% formic acid and 20 mM ammonium hexafluorophosphate with 0.1% formic acid in acetonitrile, using a gradient profile with a C18 column, exploited at 40 °C and a 1.5 mL/min flow rate. A dilute-and-shoot alternative with automated flow reversal after isocratic elution is presented. For HILIC, aqueous 100 mM ammonium formate and 0.1% formic acid in acetonitrile were used as mobile-phase components, using a gradient profile, on a Thermo Scientific™ Acclaim™ Mixed-Mode HILIC-1 column, operated at 25 °C with a 1 mL/min flow rate. UV detection was at 260 nm. Absolute limits of quantitation in the 1 μg/mL range were obtained for all tested alternatives, with 1 μL injection volumes.
尼古丁是一种广泛使用的成瘾性物质，最近也可通过电子烟获得。在此，我们展示了一项通过反相（RP）和亲水相互作用（HILIC）液相色谱法对电子烟液中的尼古丁进行的研究。针对RP机制考虑了多种水性流动相添加剂，重点关注离液剂、流动相浓度和混合比例以及柱温。样品制备通过用25 mM NaHCO/NaCO水溶液稀释的电子烟液进行甲苯液 - 液萃取。使用含0.1%甲酸的水溶液和含0.1%甲酸的20 mM六氟磷酸铵在乙腈中的混合溶液，采用梯度洗脱，在40°C下使用C18柱，以1.5 mL/min的流速，获得了保留和峰对称性的最佳RP结果。还提出了一种在等度洗脱后自动进行流动相反转的稀释进样替代方法。对于HILIC，使用含100 mM甲酸铵和0.1%甲酸的乙腈溶液作为流动相组分，采用梯度洗脱，在Thermo Scientific™ Acclaim™ Mixed-Mode HILIC-1柱上，于25°C下以1 mL/min的流速运行。紫外检测波长为260 nm。对于所有测试的替代方法，进样体积为1 μL时，在1 μg/mL范围内获得了绝对定量限。
