Vegh Caleb, Walach Gabrielle, Dube Keanna, Dobson Bromleigh, Talukdar Rohan, Wear Darcy, Jayawardena Hasana, Dufault Kaitlyn, Culmone Lauren, Srikantha Subidsa, Okaj Iva, Huggard Rachel, Cohen Jerome, Pandey Siyaram
Department of Chemistry & Biochemistry, University of Windsor, 401 Sunset Avenue, Windsor, ON N9B 3P4, Canada.
Department of Integrative Biology, University of Windsor, 401 Sunset Avenue, Windsor, ON N9B 3P4, Canada.
Nutrients. 2025 Aug 20;17(16):2701. doi: 10.3390/nu17162701.
Alzheimer's Disease (AD) is one of the most prevalent neurodegenerative disorders and the most common form of dementia. Although current treatments examine disease progression, many have side effects and primarily target symptomatic relief as opposed to halting further neurodegeneration.
The current study aims to determine the neuroprotective effects of water-soluble coenzyme Q10 (Ubisol-Q10) and an ethanolic Ashwagandha extract (E-ASH) on a transgenic mouse model of AD.
A variety of immunofluorescence staining of biomarkers was conducted to assess mechanisms commonly implicated in the disease. Additionally, spatial and non-spatial memory tests evaluated cognitive functions at two timepoints throughout the progression of the disease.
A substantial reduction in microglial activation and amyloid-β (Aβ) plaques when treated with a combination of natural health products (NHPs), Ubisol-Q10 and E-ASH. Moreover, activation of autophagy was upregulated in both the Ubisol-Q10 and combination (Ubisol-Q10+E-ASH given as a combined "Tonic" solution) groups. Oxidative stress was decreased across treated groups, while astrocyte activation was elevated in both the E-ASH and Tonic group. The Tonic group expressed an elevation in the fluorescent intensity of neuronal nuclei (NeuN) and brain-derived neurotrophic factor (BDNF) levels. Interestingly, treatment with E-ASH and Ubisol-Q10 enhanced synaptic vesicle formation compared to controls. Pre-mortem memory tests revealed the treatments to be effective at preserving cognitive abilities.
Based on these findings, the combination of E-ASH and Ubisol-Q10 may effectively mitigate the various mechanisms implicated in AD and ultimately prevent further disease progression.
阿尔茨海默病（AD）是最常见的神经退行性疾病之一，也是痴呆最常见的形式。尽管目前的治疗方法关注疾病进展，但许多治疗方法都有副作用，且主要针对症状缓解，而非阻止进一步的神经退行性变。
本研究旨在确定水溶性辅酶Q10（泛醇辅酶Q10）和印度人参乙醇提取物（E-ASH）对AD转基因小鼠模型的神经保护作用。
进行了多种生物标志物的免疫荧光染色，以评估该疾病中常见的机制。此外，在疾病进展的两个时间点进行空间和非空间记忆测试，以评估认知功能。
当用天然健康产品（NHPs）、泛醇辅酶Q10和E-ASH联合治疗时，小胶质细胞活化和β-淀粉样蛋白（Aβ）斑块显著减少。此外，泛醇辅酶Q10组和联合治疗组（泛醇辅酶Q10+E-ASH作为联合“滋补品”溶液给药）的自噬激活均上调。各治疗组的氧化应激均降低，而E-ASH组和滋补品组的星形胶质细胞活化均升高。滋补品组神经元细胞核（NeuN）的荧光强度和脑源性神经营养因子（BDNF）水平升高。有趣的是，与对照组相比，E-ASH和泛醇辅酶Q10治疗增强了突触小泡的形成。死前记忆测试表明这些治疗方法在保留认知能力方面有效。
基于这些发现，E-ASH和泛醇辅酶Q10的联合使用可能有效减轻AD中涉及的各种机制，并最终防止疾病进一步进展。
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