Liu Caiyue, Yang Xinsen, Tang Qiaoling, Cao Xiuxia, Qian Aiping, Yang Zhiwei, Wang Limin, Zhang Jianping, Pei Xinwu, Gan Lu
Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
Guyuan Branch of Ningxia Academy of Agriculture and Forestry Sciences, Guyuan 756000, China.
Plants (Basel). 2025 Aug 20;14(16):2583. doi: 10.3390/plants14162583.
Flax ( L.) is a globally important oilseed crop, valued for its edible and industrial uses. Flax seeds are rich in unsaturated fatty acids. In this study, ethyl methyl sulfone was employed to construct a mutant library from the flax cultivar Longya 10 (WT). Screening efforts identified M45, a stable mutant with an oleic acid content of 43.22% at 40 days after flowering, representing a 21.23% increase over the wild-type. RNA-Seq analysis revealed the presence of two homologs of the (stearoyl-ACP desaturase) family and two homologs of the (fatty acid desaturase 2) family, which showed differential expression in a trend consistent with the phenotype of M45. A BSA-Seq analysis was conducted to identify genes with SNPs (single nucleotide polymorphisms) and Indel (insertions/deletions) variant loci that were associated with increased oleic acid. The combination of BSA-Seq, RNA-Seq, and metabolomic analyses identified , a gene that may be co-expressed with to affect oleic acid accumulation via .
亚麻（L.）是一种全球重要的油料作物，因其食用和工业用途而受到重视。亚麻籽富含不饱和脂肪酸。在本研究中，使用乙基甲基砜从亚麻品种陇亚10（WT）构建突变体文库。筛选工作鉴定出M45，这是一个稳定的突变体，开花后40天油酸含量为43.22%，比野生型增加了21.23%。RNA-Seq分析揭示了（硬脂酰-ACP去饱和酶）家族的两个同源物和（脂肪酸去饱和酶2）家族的两个同源物的存在，它们的表达差异趋势与M45的表型一致。进行了全基因组重测序分析以鉴定与油酸增加相关的具有单核苷酸多态性（SNP）和插入/缺失（Indel）变异位点的基因。全基因组重测序、RNA-Seq和代谢组学分析的结合鉴定出一个基因，该基因可能与共同表达，通过影响油酸积累。
