Souza Guilherme de, Teixeira Clara Peleteiro, Lima Júnior Joed Pires de, Almeida Marcos Paulo Oliveira, Paschoalino Marina, Luz Luana Carvalho, Dos Santos Natália Carine Lima, de Oliveira Rafael Martins, Damasceno Izadora Santos, Barbosa Matheus Carvalho, Faria Guilherme Vieira, Ambrosio Maria Anita Lemos Vasconcelos, Veneziani Rodrigo Cassio Sola, Bastos Jairo Kenupp, Gomes Angelica Oliveira, Alves Rosiane Nascimento, Martins Carlos Henrique Gomes, Teixeira Samuel Cota, Ferro Eloisa Amália Vieira, Barbosa Bellisa Freitas
Laboratory of Immunophysiology of Reproduction, Institute of Biomedical Science, Universidade Federal de Uberlândia, Uberlândia 38405-318, MG, Brazil.
Nucleus of Research in Technological and Exact Sciences, Universidade de Franca, Franca 14404-600, SP, Brazil.
Pathogens. 2025 Jul 25;14(8):736. doi: 10.3390/pathogens14080736.
Congenital Chagas disease (CCD) is caused when crosses the placental barrier during pregnancy and reaches the fetus, which can lead to serious consequences in the developing fetus. Current treatment is carried out with nifurtimox or benznidazole, but their effectiveness is limited, and they cause side effects, requiring the search for new therapeutic strategies. In this sense, many studies have demonstrated the potential of different compounds of the genus in the control of parasitic diseases. Here, we aimed to evaluate the effect of oleoresin (OR) and leaf hydroalcoholic extract (LHE) of on infection in human villous trophoblast cells (BeWo line) and human placenta explants. Treatment with both compounds reduced invasion, proliferation, and release of trypomastigotes. Furthermore, OR and LHE affected the trypomastigotes and amastigote morphology, compromising their ability to invade and proliferate in BeWo cells, respectively. Also, treatment with OR decreased ROS production in infected BeWo cells, while LHE induced an increase. In addition, both compounds induced pro-inflammatory and anti-inflammatory cytokine production. In human placental explants, both compounds also decreased infection, in addition to inducing the production of pro-inflammatory cytokines. Thus, both OR and LHE of control infection at the human maternal-fetal interface, highlighting the possible therapeutic potential of these compounds for the treatment of CCD.
先天性恰加斯病（CCD）是在孕期 穿过胎盘屏障并到达胎儿体内时引发的，这可能会给发育中的胎儿带来严重后果。目前使用硝呋替莫或苯硝唑进行治疗，但它们的疗效有限，且会引发副作用，因此需要寻找新的治疗策略。从这个意义上说，许多研究已经证明了 属不同化合物在控制寄生虫病方面的潜力。在此，我们旨在评估 的油树脂（OR）和叶水醇提取物（LHE）对人绒毛滋养层细胞（BeWo系）和人胎盘外植体中 感染的影响。用这两种化合物进行处理均能减少锥鞭毛体的侵袭、增殖和释放。此外，OR和LHE影响锥鞭毛体和无鞭毛体的形态，分别损害它们在BeWo细胞中侵袭和增殖的能力。此外，用OR处理可降低受感染BeWo细胞中的活性氧生成，而LHE则使其增加。另外，这两种化合物均能诱导促炎和抗炎细胞因子的产生。在人胎盘外植体中，这两种化合物除了诱导促炎细胞因子的产生外，还能减少 感染。因此， 的OR和LHE均可在人母婴界面控制 感染，突出了这些化合物在治疗CCD方面可能的治疗潜力。
