Veld Luna-Faye, Waters Shelley, Lee Silvia, Hearps Anna C, Trevillyan Janine, Mushin Ari S, Foo Damien, Hoy Jennifer, Price Patricia
Curtin Medical Research Institute, Curtin Medical School, Curtin University, Bentley, WA 6102, Australia.
University of Western Australia Medical School, University of Western Australia, Nedlands, WA 6008, Australia.
Pathogens. 2025 Aug 20;14(8):822. doi: 10.3390/pathogens14080822.
Cytomegalovirus (CMV) is implicated in cardiovascular disease in healthy adults and after transplantation, but analyses in people living with HIV (PLWH) are difficult as almost all have CMV co-infections. Here, we address whether coronary artery disease (CAD) is associated with levels of CMV-reactive antibodies or with sensitivity to inflammation associated with CMV. PLWH stable on antiretroviral therapy (ART) with a recent diagnosis of CAD were matched with PLWH without CAD. Plasma samples stored at the time of the CAD event and 6, 12, 24 or 36 months earlier (n = 34-55 per group) were used for analyses. Antibodies reactive with a lysate from CMV infected cells were quantitated using an in-house ELISA, and inflammatory biomarkers were assessed using commercial kits. Bivariate analyses demonstrated similar levels of CMV antibodies in PLWH with and without CAD at all time points ( > 0.5). However, in PLWH with CAD, levels of CMV antibody correlated directly with plasma sCD14, LBP, CXCL10 and/or IL-6 at the earlier points. These correlations were not impacted by detectable plasma HIV RNA. Our findings suggest that individual differences in sensitivity to the inflammatory effects of CMV impact upon the development of CAD.
巨细胞病毒（CMV）与健康成年人及移植后的心血管疾病有关，但对艾滋病毒感染者（PLWH）的分析很困难，因为几乎所有人都合并感染了CMV。在此，我们探讨冠状动脉疾病（CAD）是否与CMV反应性抗体水平或与CMV相关的炎症敏感性有关。近期诊断为CAD且抗逆转录病毒治疗（ART）稳定的PLWH与无CAD的PLWH进行匹配。在CAD事件发生时以及6、12、24或36个月前储存的血浆样本（每组n = 34 - 55）用于分析。使用内部ELISA定量与CMV感染细胞裂解物反应的抗体，并使用商业试剂盒评估炎症生物标志物。双变量分析显示，在所有时间点，有CAD和无CAD的PLWH中CMV抗体水平相似（> 0.5）。然而，在患有CAD的PLWH中，CMV抗体水平在较早时间点与血浆sCD14、LBP、CXCL10和/或IL - 6直接相关。这些相关性不受可检测到的血浆HIV RNA的影响。我们的研究结果表明，对CMV炎症效应敏感性的个体差异会影响CAD的发生。
