Yurube Takashi, Shigemura Katsumi, Kobayashi Yurino, Maeda Taishi, Nishimura Nami, Yamada Ayaka, Kotani Kotoko, Horii Saki, Yoshida Hiroyuki, Osawa Kayo
Department of Orthopaedic Surgery, Kobe University Graduate School of Medicine, 7-5-1 Kusunoki-cho, Chuo-ku, Kobe 650-0017, Japan.
Department of Urology, Teikyo University School of Medicine, 2-11-1 Kaga, Itabashi-Ku, Tokyo 173-8605, Japan.
Pathogens. 2025 Aug 21;14(8):831. doi: 10.3390/pathogens14080831.
The DNA synthesis inhibitor zoliflodacin (ZFD) is expected to be effective against strains resistant to therapeutic agents for infection. In addition to ZFD, we investigated the susceptibility of strains to ceftriaxone (CTRX), ciprofloxacin (CPFX), garenoxacin (GRNX), and sitafloxacin (STFX). Minimum inhibitory concentration values for ZFD and four other drugs were determined for 147 strains of isolated at medical institutions in Hyogo Prefecture, Japan, from 2015 to 2022. Amino acid alterations in , , , and were examined by polymerase chain reaction and sequencing analysis. Sequence type (ST) was determined for epidemiological analysis, and strains were classified. The non-susceptibility rate was not observed in CTRX. The lowest non-susceptibility rate was observed in ZFD (39.5%) compared to CPFX (80.3%), GRNX (83.7%), and STFX (65.3%) (all < 0.0001). The most common amino acid alterations in and had non-susceptibility rates exceeding 80% to quinolones except ZFD, suggesting that these alterations may have influenced the resistance trend. STs were different between isolates in 2015 and those in 2020 and later. ZFD showed potent antimicrobial activity against strains that are highly resistant to quinolones. It may become a new option in the treatment of gonococcal infections.
DNA合成抑制剂佐利氟达辛（ZFD）有望对感染治疗药物耐药的菌株有效。除ZFD外，我们还研究了菌株对头孢曲松（CTRX）、环丙沙星（CPFX）、加替沙星（GRNX）和司帕沙星（STFX）的敏感性。测定了2015年至2022年期间从日本兵库县医疗机构分离出的147株菌株对ZFD和其他四种药物的最低抑菌浓度值。通过聚合酶链反应和测序分析检测了、、和中的氨基酸变化。确定序列类型（ST）用于流行病学分析，并对菌株进行分类。CTRX未观察到不敏感率。与CPFX（80.3%）、GRNX（83.7%）和STFX（65.3%）相比，ZFD的最低不敏感率为39.5%（所有均<0.0001）。和中最常见的氨基酸变化对除ZFD外的喹诺酮类药物的不敏感率超过80%，表明这些变化可能影响了耐药趋势。2015年分离株与2020年及以后的分离株的ST不同。ZFD对高度耐喹诺酮类的菌株显示出强大的抗菌活性。它可能成为淋病治疗的新选择。
