Porter Madison M, Davis Daniel J, McAdams Zachary L, Townsend Kile S, Martin Lynn M, Wilhite Christopher, Johnson Philip J, Ericsson Aaron C
College of Veterinary Medicine (CVM), University of Missouri (MU), 1520 E. Rollins Drive, Columbia, MO 65211, USA.
Animal Modeling Core, University of Missouri (MU), 4011 Discovery Drive, Columbia, MO 65201, USA.
Vet Sci. 2025 Jul 31;12(8):724. doi: 10.3390/vetsci12080724.
Fecal water syndrome (FWS) in horses is characterized by two-phase defecation, including both solid and liquid phases. While satisfactory explanations for FWS are unavailable, bacterial dysbiosis has been suggested as a contributing or causative factor. The objectives of this study were to determine whether fecal bacterial dysbiosis is associated with FWS in horses in the midwestern USA. Fecal samples were collected from horses with FWS and from unaffected horses at the same location. In total, 16S rRNA amplicon libraries produced from fecal bacterial DNA were sequenced using the Illumina sequencing platform. Significant differences in beta diversity were detected between affected and control horses ( = 7 × 10, F = 1.51), and differential abundance testing identified several features enriched in affected and control horses. These results agree with prior work regarding specific features in the bacterial microbiome associated with FWS, including spp., and suggest fecal dysbiosis is associated with FWS.
马的粪便水综合征（FWS）的特征是两相排便，包括固相和液相。虽然目前尚无对FWS的满意解释，但细菌群落失调被认为是一个促成因素或致病因素。本研究的目的是确定在美国中西部地区，马的粪便细菌群落失调是否与FWS有关。从患有FWS的马和同一地点未受影响的马身上采集粪便样本。总共使用Illumina测序平台对从粪便细菌DNA产生的16S rRNA扩增子文库进行测序。在受影响的马和对照马之间检测到β多样性存在显著差异（= 7×10，F = 1.51），差异丰度测试确定了在受影响的马和对照马中富集的几个特征。这些结果与之前关于与FWS相关的细菌微生物组特定特征的研究结果一致，包括 属，并表明粪便失调与FWS有关。
