Mawatari Kazuaki, Kadomura-Ishikawa Yasuko, Emoto Takahiro, Onoda Yushi, Ishida Kai, Toda Sae, Uebanso Takashi, Aizawa Toshihiko, Yamauchi Shigeharu, Fujikawa Yasuo, Tanaka Tomotake, Li Xing, Suarez-Lopez Eduardo, Kuhn Richard J, Blatchley Iii Ernest R, Takahashi Akira
Department of Preventive Environment and Nutrition, Institute of Biomedical Sciences, Tokushima University Graduate School, Tokushima 770-8503, Tokushima, Japan.
Department of Microbial Control, Institute of Biomedical Sciences, Tokushima University Graduate School, Tokushima 770-8503, Tokushima, Japan.
Viruses. 2025 Jul 30;17(8):1065. doi: 10.3390/v17081065.
Irradiation with ultraviolet light-emitting diodes (UV-LEDs) represents a promising method for viral inactivation, but a detailed understanding of the wavelength-dependent action spectra remains limited, particularly across different viral components. In this study, we established standardized UV action spectra for infectivity reduction in pathogenic viruses using a system equipped with interchangeable LEDs at 13 different peak wavelengths (250-365 nm). The reduction in viral infectivity induced by UV-LED exposure was strongly related to viral genome damage, whereas no significant degradation of viral structural proteins was detected. Peak virucidal efficiency was observed at 267-270 nm across all tested viruses, representing a slight shift from the traditionally expected 260 nm nucleic acid absorption peak. Enveloped RNA viruses, including influenza A virus, respiratory syncytial virus, and coronavirus, exhibited greater UV sensitivity than nonenveloped viruses such as feline calicivirus and adenovirus. These observations indicate that structural characteristics, such as the presence of an envelope and genome organization, influence UV susceptibility. The wavelength-specific action spectra established in this study provide critical data for optimizing UV-LED disinfection systems to achieve efficient viral inactivation while minimizing energy consumption in healthcare, food safety, and environmental sanitation.
用紫外线发光二极管（UV-LED）进行辐照是一种很有前景的病毒灭活方法，但对波长依赖性作用光谱的详细了解仍然有限，尤其是在不同病毒成分方面。在本研究中，我们使用配备了13种不同峰值波长（250-365nm）可互换LED的系统，建立了致病性病毒感染性降低的标准化紫外线作用光谱。UV-LED照射引起的病毒感染性降低与病毒基因组损伤密切相关，而未检测到病毒结构蛋白有明显降解。在所有测试病毒中，在267-270nm处观察到最高的杀病毒效率，这与传统预期的260nm核酸吸收峰略有偏移。包括甲型流感病毒、呼吸道合胞病毒和冠状病毒在内的包膜RNA病毒比诸如猫杯状病毒和腺病毒等无包膜病毒表现出更高的紫外线敏感性。这些观察结果表明，诸如包膜的存在和基因组组织等结构特征会影响紫外线敏感性。本研究中建立的波长特异性作用光谱为优化UV-LED消毒系统提供了关键数据，以便在医疗保健、食品安全和环境卫生领域实现高效病毒灭活，同时将能耗降至最低。
