Lahiri Hiya, Basu Kingshuk, Arkin Isaiah T
Department of Biological Chemistry, The Alexander Silberman Institute of Life Sciences, The Hebrew University of Jerusalem, Edmond J. Safra Campus, Jerusalem 9190400, Israel.
Department of Biomedical Engineering, City University of Hong Kong, Tat Chee Avenue, Kowloon, Hong Kong, China.
Viruses. 2025 Aug 14;17(8):1120. doi: 10.3390/v17081120.
Crimean-Congo hemorrhagic fever virus (CCHF-V) and Ebola virus are lethal pathogens that cause widespread outbreaks of hemorrhagic fever. Both diseases can be transmitted through contact with the bodily fluids of infected individuals, but as an arbovirus, CCHF-V is primarily transmitted through tick bites. Both of these viruses are classified as Risk Group 4 due to the appreciable health threat they pose. To date, there are few effective treatments available to combat these deadly hemorrhagic fevers. Consequently, identifying and characterizing ion channels (viroporins) encoded in the viral genomes may lead to potential targeted drug development. Therefore, using bacteria-based genetic assays, two viroporin candidates from CCHF-V and Ebola have been examined, and their proposed structures have been modeled to aid in further drug discovery. The results indicate that CCHF-V-gp exhibits channel activity, which is indistinguishable from established viroporins found in other viruses. In contrast, our experimental approach was unable to uncover a viroporin candidate in the Ebola virus.
克里米亚-刚果出血热病毒（CCHF-V）和埃博拉病毒是导致出血热广泛爆发的致命病原体。这两种疾病都可通过接触受感染个体的体液传播，但作为虫媒病毒，CCHF-V主要通过蜱虫叮咬传播。由于它们对健康构成的明显威胁，这两种病毒均被归类为风险等级4。迄今为止，几乎没有有效的治疗方法来对抗这些致命的出血热。因此，识别和表征病毒基因组中编码的离子通道（病毒孔蛋白）可能会带来潜在的靶向药物开发。因此，利用基于细菌的基因检测方法，对来自CCHF-V和埃博拉病毒的两种病毒孔蛋白候选物进行了研究，并对它们的推测结构进行了建模，以帮助进一步开展药物研发。结果表明，CCHF-V-gp表现出通道活性，这与其他病毒中已确定的病毒孔蛋白没有区别。相比之下，我们的实验方法未能在埃博拉病毒中发现病毒孔蛋白候选物。
