Brennan Justin W, Sun Yan
Department of Microbiology and Immunology, University of Rochester Medical Center, Rochester, NY, USA.
Methods Mol Biol. 2025;2948:29-58. doi: 10.1007/978-1-0716-4666-3_3.
Respiratory syncytial virus (RSV) is prone to generating the copy-back type of defective viral genomes (cbDVGs). cbDVGs play crucial roles in RSV pathogenesis by modulating innate immunity and directly interfering with infectious virus replication. Clinically, the timing of cbDVG emergence impacts the severity of RSV infection. DVGs are also considered a novel therapeutic approach for combating viral infections. Therefore, detecting DVGs and comprehensively understanding their functions during RSV infection are paramount. To facilitate these studies, in this chapter, we provide a summary of the commonly used methods for the detection, purification, and production of RSV cbDVGs.
呼吸道合胞病毒（RSV）易于产生回抄型缺陷病毒基因组（cbDVGs）。cbDVGs通过调节先天免疫和直接干扰传染性病毒复制，在RSV发病机制中发挥关键作用。临床上，cbDVGs出现的时间会影响RSV感染的严重程度。DVGs也被认为是对抗病毒感染的一种新型治疗方法。因此，检测DVGs并全面了解它们在RSV感染过程中的功能至关重要。为了促进这些研究，在本章中，我们总结了检测、纯化和生产RSV cbDVGs的常用方法。
