Saiardi Adolfo, Jessen Henning J, Fiedler Dorothea
Laboratory for Molecular Cell Biology, University College London, London, UK.
Institute of Organic Chemistry, Albert-Ludwigs-University of Freiburg, Freiburg, Germany.
Methods Mol Biol. 2025;2972:1-18. doi: 10.1007/978-1-0716-4799-8_1.
The diverse chemical structures of inositol pyrophosphates attract growing interest toward this class of small molecule messengers. However, their highly charged nature, the lack of a chromophore or a fluorophore, their close structural relatedness, and the complexity of their metabolism pose serious challenges to inositol pyrophosphates studies. Here, we summarize how researchers have begun to overcome these challenges and how recent experimental advances are propelling inositol pyrophosphate research into the future.
肌醇焦磷酸多样的化学结构吸引了人们对这类小分子信使越来越浓厚的兴趣。然而，它们高度带电的性质、缺乏发色团或荧光团、结构上的紧密相关性以及代谢的复杂性给肌醇焦磷酸的研究带来了严峻挑战。在此，我们总结了研究人员是如何开始克服这些挑战的，以及近期的实验进展如何推动肌醇焦磷酸研究走向未来。
