Carey Harry, Kleven Heidi, Øvsthus Martin, Yates Sharon C, Csucs Gergely, Puchades Maja A, McMullan Simon, Bjaalie Jan G, Leergaard Trygve B, Bjerke Ingvild E
Neural Systems Laboratory, Institute of Basic Medical Sciences, University of Oslo, Oslo, Norway.
Macquarie Medical School, Faculty of Medicine, Health & Human Sciences, Macquarie University, Marsfield, NSW, Australia.
Nat Commun. 2025 Aug 29;16(1):8108. doi: 10.1038/s41467-025-63177-9.
Studies of the adult mouse brain have benefited from three-dimensional (3D) atlases providing standardised frameworks for data analysis and integration. However, few 3D atlases exist for the developing mouse brain, and none cover more than a few ages. This fails to represent the dynamic nature of development and is insufficient for researchers whose data fall between the ages of existent atlases. Existing atlases also neglect the spatial correspondence between ages, making comparison across ages difficult. We present the Developmental Mouse Brain Atlas (DeMBA), a 4D atlas encompassing every postnatal day from 4 to 56. DeMBA was created by using existing brain templates for six developmental time points and interpolating intermediate ages. DeMBA is incorporated in software for spatial registration and analysis, enabling brain-wide quantification of features of interest. Furthermore, we provide the software package CCF translator, allowing coordinates or image volumes to be transformed between ages for comparison. These resources provide age-specific spatial frameworks incorporated in accessible analytic pipelines for developmental mouse brain data.
对成年小鼠大脑的研究受益于三维（3D）图谱，这些图谱为数据分析和整合提供了标准化框架。然而，针对发育中小鼠大脑的3D图谱却很少，而且没有一个涵盖超过几个年龄阶段。这无法体现发育的动态本质，对于那些数据处于现有图谱年龄范围之间的研究人员来说是不够的。现有的图谱还忽略了不同年龄之间的空间对应关系，使得跨年龄比较变得困难。我们展示了发育小鼠脑图谱（DeMBA），这是一个涵盖出生后第4天到第56天每一天的四维图谱。DeMBA是通过使用六个发育时间点的现有脑模板并插入中间年龄创建的。DeMBA被整合到用于空间配准和分析的软件中，能够对感兴趣的特征进行全脑定量分析。此外，我们提供了软件包CCF翻译器，允许在不同年龄之间转换坐标或图像体积以进行比较。这些资源提供了特定年龄的空间框架，并融入了便于获取的发育小鼠脑数据的分析流程中。
