Bais Gabrielle B, Giudice Jimena
Curriculum in Cell Biology and Physiology, The University of North Carolina at Chapel Hill, Chapel Hill, NC, USA; Department of Cell Biology and Physiology, The University of North Carolina at Chapel Hill, Chapel Hill, NC, USA; Integrated Vascular Biology Training Program, The University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.
Curriculum in Cell Biology and Physiology, The University of North Carolina at Chapel Hill, Chapel Hill, NC, USA; Department of Cell Biology and Physiology, The University of North Carolina at Chapel Hill, Chapel Hill, NC, USA; McAllister Heart Institute, The University of North Carolina at Chapel Hill, Chapel Hill, NC, USA; RNA Discovery Center, The University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.
Cell Rep. 2025 Sep 23;44(9):116175. doi: 10.1016/j.celrep.2025.116175. Epub 2025 Aug 29.
Through mechanotransduction, cells sense and respond to mechanical stimuli from their environment. A mechanical stimulus is first detected by a mechanosensor, then converted into a biochemical signal, which can ultimately control the expression of genes. RNA processing, which includes canonical and alternative splicing, 3' end polyadenylation, and 5' end capping, is a mechanism that fine-tunes gene expression regulation. Here, we provide an overview of recent studies revealing substantial links between mechanotransduction and RNA processing, with a focus on alternative splicing and polyadenylation. We first describe the molecular players that are known to mediate mechanotransduction. Then, we examine how mechanical forces inform the functions of RNA-binding proteins. We next summarize recent investigations demonstrating that genes encoding mechanosensory proteins are alternatively spliced and how alternative splicing might impact isoform functions. Last, we discuss the role of mechanical forces on alternative splicing and polyadenylation landscapes.
通过机械转导，细胞感知并响应来自其环境的机械刺激。机械刺激首先由机械传感器检测到，然后转化为生化信号，最终可控制基因表达。RNA加工，包括经典剪接和可变剪接、3'端聚腺苷酸化以及5'端加帽，是一种微调基因表达调控的机制。在此，我们概述了近期的研究，这些研究揭示了机械转导与RNA加工之间的实质性联系，重点关注可变剪接和聚腺苷酸化。我们首先描述已知介导机械转导的分子参与者。然后，我们研究机械力如何影响RNA结合蛋白的功能。接下来，我们总结近期的研究，这些研究表明编码机械传感蛋白的基因存在可变剪接，以及可变剪接可能如何影响异构体功能。最后，我们讨论机械力在可变剪接和聚腺苷酸化格局中的作用。
