Zhang Qian, Zhu Ying, Li Junjie, Duan Mengxue, Zhu Hongqiu
Clinical Medical College, Chengdu University of Traditional Chinese Medicine, Chengdu, People's Republic of China.
College of Medicine and Life Sciences, Chengdu University of Traditional Chinese Medicine, Chengdu, People's Republic of China.
Int J Gen Med. 2025 Aug 27;18:4851-4862. doi: 10.2147/IJGM.S530104. eCollection 2025.
To investigate the therapeutic effects of Bushen Huoxue Formula combined with aerobic exercise (COMB) on reproductive function in obese polycystic ovary syndrome (PCOS) mice.
Seventy-six 3-week-old female C57BL/6JGpt mice were randomly divided into a control group (CON, =14) and a model group (MOD, =62). The PCOS model was established using a high-fat diet (HFD) combined with dehydroepiandrosterone (DHEA). Successfully modeled mice were randomly allocated into the MOD group (=12), metformin group (MET, =12), Bushen Huoxue Formula group (BHF, =12), aerobic exercise group (AE, =12), and BHF combined with AE group (COMB, =12). After 8 weeks of intervention, 6 female mice were randomly selected from each group. Body weight, body fat percentage (BFP), and fasting plasma glucose (FPG) were measured. Fasting insulin (FINS) and serum sex hormone levels (follicle-stimulating hormone (FSH), luteinizing hormone (LH), testosterone (T), and estradiol (E2)) were determined using enzyme-linked immunosorbent assay (ELISA). The remaining female mice in each group were co-housed with 10-week-old male mice. Pregnancy rate, abortion rate, and embryonic resorption rate were recorded.
After 8 weeks of intervention, MET, BHF, AE, and COMB groups showed significant improvements in ovarian morphology, body weight, BFP, insulin resistance (HOMA-IR), and serum sex hormone levels (P<0.05). Compared with the BHF and AE groups, the COMB group demonstrated superior effects in reducing BFP, T levels, and HOMA-IR (P<0.05). All intervention groups showed higher pregnancy rates compared to the MOD group. Notably, the BHF, AE, and COMB groups exhibited lower abortion and embryo loss rates than the MET group. COMB group showed the most favorable pregnancy outcomes, including higher pregnancy rate, lower abortion and embryo absorption rates, and increased total number of embryos.
The combined intervention of the COMB group demonstrated superior therapeutic effects on reproductive function in obese PCOS mice compared to the individual interventions of the BHF group or AE group, with a better safety profile. The integrated sports-medicine approach shows promising clinical potential.
探讨补肾活血方联合有氧运动（COMB）对肥胖型多囊卵巢综合征（PCOS）小鼠生殖功能的治疗作用。
将76只3周龄雌性C57BL/6JGpt小鼠随机分为对照组（CON，n = 14）和模型组（MOD，n = 62）。采用高脂饮食（HFD）联合脱氢表雄酮（DHEA）建立PCOS模型。将造模成功的小鼠随机分为模型组（n = 12）、二甲双胍组（MET，n = 12）、补肾活血方组（BHF，n = 12）、有氧运动组（AE，n = 12）和补肾活血方联合有氧运动组（COMB，n = 12）。干预8周后，每组随机选取6只雌性小鼠，测量体重、体脂百分比（BFP）和空腹血糖（FPG）。采用酶联免疫吸附测定（ELISA）法测定空腹胰岛素（FINS）和血清性激素水平（促卵泡生成素（FSH）、促黄体生成素（LH）、睾酮（T）和雌二醇（E2））。每组剩余的雌性小鼠与10周龄雄性小鼠合笼饲养，记录妊娠率、流产率和胚胎吸收率。
干预8周后，MET组、BHF组、AE组和COMB组的卵巢形态、体重、BFP、胰岛素抵抗（HOMA-IR）和血清性激素水平均有显著改善（P<0.05）。与BHF组和AE组相比，COMB组在降低BFP、T水平和HOMA-IR方面效果更优（P<0.05）。与MOD组相比，所有干预组的妊娠率均较高。值得注意的是，BHF组、AE组和COMB组的流产和胚胎丢失率低于MET组。COMB组的妊娠结局最佳，包括妊娠率较高、流产和胚胎吸收率较低以及胚胎总数增加。
与BHF组或AE组的单独干预相比，COMB组的联合干预对肥胖型PCOS小鼠的生殖功能具有更优的治疗效果，且安全性更好。综合运动医学方法显示出有前景的临床潜力。
