Han Wenjiao, Han Yang, Ma Yanru, Yu Guolei, Jiang Xushi, Yang Qian, Liu Na, Wang Caixia, Li Baohua, Lian Sen, Ren Weichao
Shandong Engineering Research Center for Environment-Friendly Agricultural Pest Management, College of Plant Health and Medicine, Qingdao Agricultural University, Qingdao, China.
Front Plant Sci. 2025 Aug 15;16:1590359. doi: 10.3389/fpls.2025.1590359. eCollection 2025.
Autophagy is a fundamental cellular degradation and recycling system that is crucial for maintaining cellular homeostasis, responding to stress, and ensuring the proper functioning of cells. To date, the biological functions of autophagy in the plant-pathogenic fungus remains largely unknown. In this study, we identified and characterized the E2-like enzyme Atg3 in . The autophagic process was blocked in the deletion mutant Δ, and the Δ mutant showed serious defects in mycelial growth, conidiation, perithecium formation and virulence. In addition, the Δ mutant exhibited an increased number of nuclei in mycelial compartment. All of the phenotypic changes of the Δ mutant were restored by gene complementation. These results indicate that the E2-like enzyme Atg3 plays an important role in various developmental processes and pathogenesis of , which provides a potential target for developing novel fungicides.
自噬是一种基本的细胞降解和循环利用系统，对于维持细胞内稳态、应对压力以及确保细胞正常功能至关重要。迄今为止，自噬在植物致病真菌中的生物学功能仍 largely未知。在本研究中，我们鉴定并表征了中的类E2酶Atg3。自噬过程在缺失突变体Δ中受阻，Δ突变体在菌丝生长、分生孢子形成、子囊壳形成和毒力方面表现出严重缺陷。此外，Δ突变体在菌丝隔室中的细胞核数量增加。基因互补恢复了Δ突变体的所有表型变化。这些结果表明，类E2酶Atg3在的各种发育过程和致病机制中起重要作用，这为开发新型杀菌剂提供了潜在靶点。
