Shandong Engineering Research Center for Environment-Friendly Agricultural Pest Management, College of Plant Health and Medicine, Qingdao Agricultural University, Qingdao 266109, China.
Shandong Engineering Research Center for Environment-Friendly Agricultural Pest Management, College of Plant Health and Medicine, Qingdao Agricultural University, Qingdao 266109, China.
Pestic Biochem Physiol. 2024 Nov;205:106134. doi: 10.1016/j.pestbp.2024.106134. Epub 2024 Sep 17.
Histidine kinases (HKs) allow fungal cells to sense and respond to environmental stimuli. However, the biological role of HKs in Botryosphaeria dothidea, the causal agent of Botryosphaeria canker and apple ring rot, remains unknown. In this study, we identified and characterized the two-component histidine kinase BdHk1 in B. dothidea. Targeted knockout of BdHK1 gene resulted in severe conidiation and pathogenicity defects. In addition, the ΔBdHk1 mutant showed hypersensitivity to osmotic stress, but resistance to phenylpyrrole and dicarboximide fungicides. Moreover, the ΔBdHk1 mutant exhibited significantly increased sensitivity to the cell membrane-damaging agent SDS and high temperature. Comparative transcriptome analysis revealed that inactivation of BdHk1 influenced multiple metabolic pathways in B. dothidea. Taken together, our results suggest that BdHk1 plays an important role in development, virulence and stress tolerance in B. dothidea.
组氨酸激酶 (HKs) 使真菌细胞能够感知和响应环境刺激。然而，HKs 在 Botryosphaeria dothidea（引起苹果溃疡病和苹果轮纹病的病原体）中的生物学作用尚不清楚。在这项研究中，我们鉴定并表征了 Botryosphaeria dothidea 中的双组分组氨酸激酶 BdHk1。BdHK1 基因的靶向敲除导致严重的分生孢子形成和致病性缺陷。此外，ΔBdHk1 突变体对渗透压胁迫表现出超敏性，但对苯并吡咯和二羧酰亚胺类杀菌剂具有抗性。此外，ΔBdHk1 突变体对细胞膜损伤剂 SDS 和高温的敏感性显著增加。比较转录组分析表明，BdHk1 的失活影响了 Botryosphaeria dothidea 中的多个代谢途径。总之，我们的结果表明，BdHk1 在 Botryosphaeria dothidea 的发育、毒力和应激耐受中发挥重要作用。
