Selmayr E, Müller-Berghaus G
Thromb Haemost. 1985 Dec 17;54(4):804-7.
The present study is concerned with the formation of fibrin-fibrinogen associations in the presence of FXIIIa while fibrinolysis was inhibited by aprotinin and EACA. SDS agarose-polyacrylamide gel electrophoresis on 2.5% gels under non-reducing conditions and ultracentrifugation of the associations on urea-sucrose density gradients showed the formation of soluble cross-linked high molecular weight (HMW) fibrin-fibrinogen polymers with an estimated molecular size up to 10 times that of fibrinogen. After incubation of a mixture of 131I-fibrinogen (4 mg/ml) and 125I-desAA-fibrin (0.2 mg/ml) with FXIIIa (2 U/ml) for 1 h at 37 degrees C, about 5% of the fibrinogen and 80% of the fibrin was incorporated into the generated soluble HMW polymers. The detection of soluble crosslinked fibrin-fibrinogen polymers could be a useful diagnostic criterion for imminent DIC.
本研究关注在凝血因子ⅩⅢa存在的情况下纤维蛋白-纤维蛋白原缔合物的形成，同时纤溶酶原激活物抑制剂和6-氨基己酸抑制纤维蛋白溶解。在非还原条件下于2.5%凝胶上进行SDS琼脂糖-聚丙烯酰胺凝胶电泳，以及在尿素-蔗糖密度梯度上对缔合物进行超速离心，结果显示形成了可溶性交联高分子量（HMW）纤维蛋白-纤维蛋白原聚合物，其估计分子大小是纤维蛋白原的10倍。将131I-纤维蛋白原（4mg/ml）和125I-去氨基端纤维蛋白（DEAA-fibrin，0.2mg/ml）的混合物与凝血因子ⅩⅢa（2U/ml）在37℃孵育1小时后，约5%的纤维蛋白原和80%的纤维蛋白被整合到生成的可溶性HMW聚合物中。可溶性交联纤维蛋白-纤维蛋白原聚合物的检测可能是即将发生弥散性血管内凝血（DIC）的有用诊断标准。
