Ricci G, Manservigi R, Albonici L, Zavagli G, Cassai E
Thromb Haemost. 1985 Dec 17;54(4):862-5.
In the present research protein analysis on SDS-polyacrylamide gel electrophoresis (PAGE) has been used to study the glycoprotein pattern of the blood platelets of four members from a family affected by May-Hegglin anomaly. In order to characterize the glycoprotein fractions, lactoperoxidase-iodination and immunoprecipitation procedures were used. N,N'diallyltartardiamide (DATD) cross-linked gel electrophoresis was shown to improve the glycoprotein pattern resolution, although with the lactoperoxidase-iodination the glycoprotein characterization of May-Hegglin platelets overlapped the normal. On the other hand with the immunoprecipitation the specific antiserum precipitated all the five major fractions of normal membrane glycoproteins, but it was shown to react quite poorly with the component V of the May-Hegglin glycoprotein pattern.
在本研究中，利用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（PAGE）进行蛋白质分析，以研究一个受May-Hegglin异常影响的家族中四名成员血小板的糖蛋白模式。为了表征糖蛋白组分，采用了乳过氧化物酶碘化和免疫沉淀程序。结果表明，N，N'-二烯丙基酒石酰胺（DATD）交联凝胶电泳可提高糖蛋白模式的分辨率，不过在乳过氧化物酶碘化过程中，May-Hegglin血小板的糖蛋白特征与正常情况重叠。另一方面，通过免疫沉淀，特异性抗血清沉淀出了正常膜糖蛋白的所有五个主要组分，但结果显示其与May-Hegglin糖蛋白模式的组分V反应很差。
