Hatanaka K, Minamiyama M, Takaichi S, Tanaka K, Ishibashi-Ueda H, Imakita M, Yamamoto A
Thromb Res. 1985 Dec 15;40(6):731-43. doi: 10.1016/0049-3848(85)90311-1.
Mesenteries of mice under anesthesia were stretched over an inverted microscope. A micropipette filled with solution containing various concentrations of ADP or thrombin was brought into contact with the outside of a mesenteric vein by micromanipulation, and then poured over the outer surface of the vein. Morphological characteristics of the thrombi and the time needed for thrombus formation were examined. Application of either thrombin or ADP to the adventitia of mesenteric veins caused thrombus formation. Although thrombi by application of ADP seemed to be anchored by direct adhesion of platelets to the exposed subendothelium, thrombi by application of thrombin seemed to be anchored by deposited fibrin.
将处于麻醉状态的小鼠肠系膜铺展在倒置显微镜上。通过显微操作，将一支充满含有不同浓度ADP或凝血酶溶液的微量移液器与肠系膜静脉外部接触，然后将溶液浇在静脉外表面。检查血栓的形态特征以及形成血栓所需的时间。将凝血酶或ADP应用于肠系膜静脉外膜均会导致血栓形成。尽管应用ADP形成的血栓似乎是通过血小板直接粘附于暴露的内皮下而固定，但应用凝血酶形成的血栓似乎是通过沉积的纤维蛋白而固定。
