Sun Bolu, Kang Jiali, Wang Zixia, Wu Yuqiong, He Haiying, Wang Xinlan, Da Xuanxiu, Zhou Miao, Lv Ying, Qin Chenyu, Wang Xiangdong, Yang Lin, Li Huajie, Wang Hai
School of Life Science and Engineering, Key Laboratory of Herbal-Tibetan Drug Screening and Deep Processing of Gansu Province, Gansu Industrial Technology Center for Food and Pharmaceutical Resources Development and Biomanufacturing, Lanzhou University of Technology, Lanzhou, Gansu, 730050, China.
Department of Pharmacy, Affiliated Hospital of Yangzhou University, Yangzhou, 225012, Jiangsu, China.
Mikrochim Acta. 2025 Sep 3;192(10):629. doi: 10.1007/s00604-025-07420-6.
An electrochemical immunosensor was successfully developed for evaluating traditional Chinese medicine (TCM)-induced cardiac toxicity by immobilizing heart-type fatty acid-binding protein (HFABP)-specific antibodies on an electrode surface modified with chitosan (CS)-functionalized metal-organic framework ZIF-8 and gold nanoparticles (AuNPs). Leveraging the excellent biocompatibility of AuNPs, the excellent stability and high surface area of ZIF-8, along with the strong specific recognition capability of HFABP antibodies, the sensor demonstrated superior performance. Under optimized experimental conditions, it achieved an ultra-low detection limit of 7.09 × 10 pg mL for HFABP, maintained excellent linearity in the range 1.57 × 10 ~ 1.60 × 10 ng mL, and outperformed conventional ELISA methods in sensitivity when applied to TCM-induced cardiac toxicity models. The sensor also demonstrated excellent specificity, reproducibility, and stability. This research provides a sensitive and rapid new method for early warning of TCM-induced cardiac toxicity and contributes technical support and scientific reference for improving diagnostic accuracy in herbal medicine safety assessment.
通过将心脏型脂肪酸结合蛋白（HFABP）特异性抗体固定在壳聚糖（CS）功能化的金属有机框架ZIF-8和金纳米颗粒（AuNPs）修饰的电极表面，成功开发了一种电化学免疫传感器，用于评估中药（TCM）引起的心脏毒性。利用AuNPs优异的生物相容性、ZIF-8优异的稳定性和高比表面积，以及HFABP抗体强大的特异性识别能力，该传感器表现出卓越的性能。在优化的实验条件下，它对HFABP实现了7.09×10 pg mL的超低检测限，在1.57×10～1.60×10 ng mL范围内保持了优异的线性，并且在应用于中药引起的心脏毒性模型时，其灵敏度优于传统酶联免疫吸附测定（ELISA）方法。该传感器还表现出优异的特异性、重现性和稳定性。本研究为中药引起的心脏毒性早期预警提供了一种灵敏、快速的新方法，并为提高草药安全性评估中的诊断准确性提供了技术支持和科学参考。
