Park Ji Hyeon, Shin Jae Young, Che Denis Nchang, Kim Mi Yeung, Hur Yong Kap, Song Geun Seoup, Cho Byoung Ok, Jang Seon Il
Research Institute, Unique Biotech Co., Ltd., Iksan, Jeonbuk 54576, Republic of Korea.
Institute of Health Science, Jeonju University, Jeonju, Jeonbuk 55069, Republic of Korea.
Exp Ther Med. 2025 Aug 14;30(4):200. doi: 10.3892/etm.2025.12950. eCollection 2025 Oct.
Airway inflammation driven by particulate matter (PM) exposure underlies diseases such as asthma and allergic rhinitis. Although conventional anti-inflammatory therapies exist, they often cause significant side effects. Natural plant extracts offer non-toxic alternatives with comparable efficacy. The present study evaluated the effects of -propolis (PJP) mixture in a mouse model co-exposed to PM (intranasal) and ovalbumin (OVA; intraperitoneal) over 30 days. PJP was administered orally at 50, 100 or 200 mg/kg daily for 9 days. PJP reduced sneezing and nasal rubbing. Serum levels of total IgE and OVA-specific IgG were decreased by PJP. In addition, bronchoalveolar lavage fluid and nasal lavage fluid showed lower histamine and IL-4 concentrations. In lung tissue, PJP reduced the epithelial thickness and inflammatory cell infiltration (goblet cells, eosinophils and mast cells). At the molecular level, PJP downregulated suppression of tumorigenicity 2, IL-33, TNF-α and IL-4 expression, and inhibited NF-κB phosphorylation. PJP attenuated PM/OVA-induced airway inflammation by suppressing NF-κB signaling and associated cytokine responses, highlighting its potential as a therapeutic candidate for inflammatory respiratory diseases.
暴露于颗粒物（PM）引发的气道炎症是哮喘和过敏性鼻炎等疾病的基础。尽管存在传统的抗炎疗法，但它们常常会引起显著的副作用。天然植物提取物提供了具有相当疗效的无毒替代方案。本研究评估了巴西绿蜂胶（PJP）混合物在小鼠模型中对30天内同时经鼻暴露于PM和腹腔注射卵清蛋白（OVA）的影响。PJP以50、100或200mg/kg的剂量每日口服给药9天。PJP减少了打喷嚏和擦鼻行为。PJP降低了血清总IgE和OVA特异性IgG水平。此外，支气管肺泡灌洗液和鼻腔灌洗液中的组胺和IL-4浓度较低。在肺组织中，PJP减少了上皮厚度和炎症细胞浸润（杯状细胞、嗜酸性粒细胞和肥大细胞）。在分子水平上，PJP下调了肿瘤抑制因子2、IL-33、TNF-α和IL-4的表达，并抑制了NF-κB磷酸化。PJP通过抑制NF-κB信号传导和相关细胞因子反应减轻了PM/OVA诱导的气道炎症，突出了其作为炎症性呼吸道疾病治疗候选药物的潜力。
