Ahmad Karam, Ali Mhammad, Muhammad Suliman, Daoud Ali, Khalil Abdulkarim
Department of Oral and Maxillofacial Surgery, Tishreen University Hospital, Lattakia, Syria.
Faculty of Dentistry, Al-Andalus University for Medical Sciences, Tartus, Syria.
Ann Med Surg (Lond). 2025 Jul 14;87(9):5503-5511. doi: 10.1097/MS9.0000000000003581. eCollection 2025 Sep.
The purpose of this study is to evaluate the effectiveness of simvastatin in the bone formation of osteoporotic mandible in the rat model.
Eight Wistar male rats at the age of 6 months, with a weighted average of 250-300 grams, were purchased from the Atomic Energy Authority in Damascus (Syria). Osteoporosis was induced through bilateral orchiectomy, a procedure that involves the removal of the testes, under anesthesia with a combination of ketamine and xylazine. The lateral mandibular portion was selected to create a physiologically critical bone defect. In each rat, two adjacent defects were created in the mandible, and simvastatin gel 1% was applied to one side of them. The rats were euthanized over the first 3 months, and the defective portions of the mandible were processed for histological analysis of formed bone.
The study used Allen's scoring system to measure the amount of new bone formed in the defects treated with simvastatin compared to untreated defects. During the first and second months, the simvastatin-treated defects showed significantly higher bone formation than the untreated defects (P < 0.05), indicating that simvastatin had a positive impact on bone healing in the early stages. However, by the third month, this difference disappeared (P > 0.05), suggesting that simvastatin's effect on bone formation was strongest early on and did not provide additional benefits over time.
Our findings suggest that local application of simvastatin (1% gel) has favorable effects on bone formation in osteoporotic mandibular rats.
本研究的目的是评估辛伐他汀对大鼠骨质疏松性下颌骨骨形成的有效性。
从叙利亚大马士革的原子能机构购买了8只6个月大的雄性Wistar大鼠，平均体重250 - 300克。通过双侧睾丸切除术诱导骨质疏松，该手术在氯胺酮和赛拉嗪联合麻醉下进行，涉及切除睾丸。选择下颌骨外侧部分制造生理性关键骨缺损。在每只大鼠的下颌骨中制造两个相邻的缺损，其中一侧应用1%的辛伐他汀凝胶。在最初3个月内对大鼠实施安乐死，并对下颌骨的缺损部分进行处理，以对形成的骨进行组织学分析。
该研究使用艾伦评分系统来测量与未治疗的缺损相比，用辛伐他汀治疗的缺损中形成的新骨量。在第一个月和第二个月期间，用辛伐他汀治疗的缺损显示出比未治疗的缺损明显更高的骨形成（P < 0.05），表明辛伐他汀在早期对骨愈合有积极影响。然而，到第三个月时，这种差异消失了（P > 0.05），这表明辛伐他汀对骨形成的作用在早期最强，随着时间的推移没有提供额外的益处。
我们的研究结果表明，局部应用辛伐他汀（1%凝胶）对骨质疏松性下颌大鼠的骨形成有有利影响。
