Das Papri, Murthy Smrithi, Abbas Eshan, White Kristin, Arya Richa
Cytogenetics Laboratory, Department of Zoology, Institute of Science, Banaras Hindu University, Varanasi-221005.
Ashanagar Phase 2, Mulund (West), Mumbai 400080.
Mol Biol Cell. 2025 Sep 3:mbcE24080347. doi: 10.1091/mbc.E24-08-0347.
Cell size is strongly correlated with several biological processes, including the cell cycle and growth. Here, we investigated the regulation of stem cell size during central nervous system (CNS) development and its association with cell fate. We note that neural stem cells (NSCs) in different regions of the ventral nerve cord increase their size at different rates. Thoracic NSCs grow at a faster rate compared to those in the abdominal region during larval development. We show that in addition to the known role in apoptosis and nervous system remodeling, larval expression of is crucial in regulating the rate of postembryonic NSCs size increase, their timely exit from G2 phase and mitotic rate. We demonstrate that when expression is lost in abdominal NSCs, their size increases, they exhibit a shorter G2 phase, enter mitosis earlier, and divide more rapidly. Conversely, the introduction of in thoracic NSCs slows their growth and delays their entry into mitosis. We demonstrate that -mediated NSC size regulation acts downstream of their nutrition-induced activation, thereby fine-tuning the stem cell potential spatiotemporally. This study highlights the instructive role of in regulating various fates of larval NSCs during CNS patterning.
细胞大小与包括细胞周期和生长在内的多个生物学过程密切相关。在此，我们研究了中枢神经系统（CNS）发育过程中干细胞大小的调控及其与细胞命运的关联。我们注意到腹侧神经索不同区域的神经干细胞（NSCs）以不同速率增大其大小。在幼虫发育期间，胸部神经干细胞相比于腹部区域的神经干细胞生长速率更快。我们表明，除了在细胞凋亡和神经系统重塑中的已知作用外，幼虫期 的表达对于调节胚后神经干细胞大小增加的速率、它们及时退出G2期以及有丝分裂速率至关重要。我们证明，当腹部神经干细胞中 表达缺失时，它们的大小增加，表现出更短的G2期，更早进入有丝分裂，并且分裂更快。相反，在胸部神经干细胞中引入 会减缓它们的生长并延迟它们进入有丝分裂。我们证明， -介导的神经干细胞大小调控作用于营养诱导激活的下游，从而在时空上微调干细胞潜能。这项研究突出了 在中枢神经系统模式形成过程中调节幼虫神经干细胞各种命运方面的指导作用。
