Su Yang, Callender Maiya, Woolsey Dylan, Zhu Joanna, Sedney Colleen, Vu Mimi Thu, Masters Jillian, Caulfield Amanda, Dewan Kalyan, Gaskill Emily, Stevens Reilly, Joshua Austin, Evans Christopher M, Glushka John, Vinogradov Evgeny, Preston Andrew, Krunkosky Thomas, Bar-Peled Maor, Harvill Eric T
Department of Biochemistry and Molecular Biology, University of Georgia, Athens, GA, USA.
Complex Carbohydrate Research Center, University of Georgia, Athens, GA, USA.
Sci Adv. 2025 Sep 5;11(36):eadw7764. doi: 10.1126/sciadv.adw7764. Epub 2025 Sep 3.
Respiratory tract infections pose considerable global health challenges. Upper airway colonization is pivotal to these infections, including those caused by species. We identified an oligosaccharide, bordetellae colonization oligosaccharide (b-Cool), crucial for early nasal colonization of . We characterized the structure of b-Cool by LC-MS and NMR and found that it is prevalent across a diverse range of bordetellae, including , which causes whooping cough in humans. A mutant lacking b-Cool (Δ) showed significantly delayed and decreased colonization in mouse nasopharynx and nasal epithelia, resulting in decreased transmission. The colonization defect of Δ was rescued in mucin deficient mice, suggesting that b-Cool may facilitate colonization in the presence of airway mucins.
呼吸道感染给全球健康带来了巨大挑战。上呼吸道定植对于这些感染至关重要，包括由某些物种引起的感染。我们鉴定出一种寡糖，即博德特氏菌定植寡糖（b-Cool），它对于[具体物种]的早期鼻腔定植至关重要。我们通过液相色谱-质谱联用（LC-MS）和核磁共振（NMR）对b-Cool的结构进行了表征，发现它在多种博德特氏菌中普遍存在，包括在人类中引起百日咳的[具体物种]。一个缺乏b-Cool的突变体（Δ）在小鼠鼻咽和鼻上皮中的定植明显延迟且减少，导致传播减少。在黏蛋白缺陷小鼠中，Δ的定植缺陷得到了挽救，这表明b-Cool可能在气道黏蛋白存在的情况下促进定植。
