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[通过淋巴细胞和裂解红细胞的细胞培养物感染来证明猪瘟病毒]

[Demonstration of the swine fever virus by the infection of cell cultures of lymphocytes and lysed red blood cells].

作者信息

Chenchev I, Milev N, Peev Ia, Aleksandov M

出版信息

Vet Med Nauki. 1985;22(10):16-9.

PMID:4090264
Abstract

A method was worked out for the diagnosing of swine fever by means of infecting sensitive cell cultures on lamellae with lyzed red blood cells and lymphocytes, the demonstration being performed through immunofluorescence. The new method made it possible to avoid killing of suspected animals for diagnostic purposes (in order to prepare suspensions of parenchymal organs and isolate the virus) while the characteristic morphologic changes were still not well manifested.

摘要

制定了一种通过用裂解的红细胞和淋巴细胞感染薄片上的敏感细胞培养物来诊断猪瘟的方法,通过免疫荧光进行验证。这种新方法能够避免为诊断目的而宰杀疑似动物(以便制备实质器官悬液并分离病毒),而此时特征性形态变化尚未充分显现。

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